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Article
January 1986

Antigenic and Cellular Heterogeneity of Primary Uveal Malignant Melanomas

Author Affiliations

From the Department of Clinical Research of the Retina Service (Dr Donoso and Ms Whitman), the Oncology Service (Drs Shields, Augsburger, and Donoso), and the Department of Pathology (Dr Shields and Ms Arbizo), Wills Eye Hospital and Thomas Jefferson University, Philadelphia.

Arch Ophthalmol. 1986;104(1):106-110. doi:10.1001/archopht.1986.01050130120034
Abstract

• Monoclonal antibody (MAb) MAb8-1H, which identifies an ocular melanoma— associated antigen, and computerassisted cytomorphometric analysis were used to investigate antigenic and cellular heterogeneity in four atypical cases of choroidal or ciliary body melanoma. The presence of one or more well-localized, morphologically distinct areas within each uveal melanoma, suggestive of multiple tumor cell populations, was observed by conventional light microscopy. In one choroidal melanoma with an unusual bilobed configuration, MAb8-1H bound only to the anterior lobe composed of spindle melanoma cells. In the other three cases, MAb8-1H bound selectively to discrete regions within the uveal melanoma corresponding to the well-localized, morphologically distinct areas seen by light microscopy. In each case, a computer was used to calculate the nuclear area of 50 tumor cells corresponding to the areas binding or not binding MAb8-1H. In three of the four cases examined, the differences in mean nuclear area between the discrete populations of tumor cells were statistically significant. Our results suggest that some uveal melanomas are composed of two or more discrete populations of tumor cells with regard to both expression of melanoma-associated antigens and cytomorphologic features.

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