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Article
January 1986

Rhodopsin and RetinoblastomaA Monoclonal Antibody Histopathologic Study

Author Affiliations

From the Retina Service, the Oncology Service, and the Department of Pathology, Wills Eye Hospital, Philadelphia, (Drs Donoso, Augsburger, and Shields and Ms Arbizo) the Department of Biochemistry the Wistar Institute for Anatomy and Biology, Philadelphia (Dr Dietzschold), and the Department of Physiology and Biophysics, University of Illinois, Chicago (Dr Hamm).

Arch Ophthalmol. 1986;104(1):111-113. doi:10.1001/archopht.1986.01050130125035
Abstract

• Rhodopsin was identified in formaldehyde-fixed, paraffin-embedded human fetal retina, and in five retinoblastomas using monoclonal antibody (MAb) MAb-E. The binding pattern corresponding to rhodopsin immunoreactivity was then compared with S-antigen using another monoclonal antibody, MAbA9-C6. Rhodopsin and S-antigen were first observed in the 18-week-old human fetal eye, at a stage preceding photoreceptor differentiation. In adult eyes containing normal photoreceptor cells, rhodopsin immunoreactivity was restricted to the rod outer segments, whereas S-antigen immunoreactivity was localized to the entire photoreceptor cell. In retinoblastomas both monoclonal antibodies bound to the same area of the tumor; however, different and distinct staining patterns associated with each monoclonal antibody were recognized. In four cases, an intense well-circumscribed "halo" pattern, characteristic of cell-surface binding, was associated with rhodopsin, whereas the binding pattern associated with S-antigen was intense, well localized, and cytoplasmic in all cases. Our results show that some well-differentiated retinoblastomas express both rhodopsin and S-antigen, and as such express proteins that participate in the initial events in the phototransduction of vision.

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