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January 1986

Susceptibility of Acanthamoeba to Cryotherapeutic Method

Author Affiliations

From the Departments of Ophthalmology (Drs Meisler, Ludwig, and Langston) and Microbiology (Dr Rutherford and Mr Bican), The Cleveland Clinic Foundation, and the Centers for Disease Control, Atlanta (Dr Visvesvara).

Arch Ophthalmol. 1986;104(1):130-131. doi:10.1001/archopht.1986.01050130144040

Acanthamoeba keratitis is becoming an increasingly well-known clinical entity. The Acanthamoeba species in their encysted state are resistant to antimicrobial agents, resulting in corneal infections that are refractory to medical therapy. Corneal cryotherapy has been used to treat Acanthamoeba keratitis with varying and equivocal success. We subjected trophozoites and cysts of two species of Acanthamoeba recovered from corneal infections to trials of in vitro freeze-thawrefreeze cryotherapeutic methods. The trophozoites of both species were killed in all trials. However, the cysts of both species survived all trials. Cryotherapy may not be an effective means to eliminate Acanthamoeba cysts from the cornea.