• A recently developed direct-smear fluorescent antibody (DFA) cytology method of detecting chlamydial infection was evaluated in an area where trachoma is endemic. Four hundred seventy-five children aged 2 to 10 years were examined, and adequate conjunctival cytology specimens were collected from 457 (96%). Trachomatous inflammatory changes were observed in 114 (25%) of the children. The DFA cytologic technique has a high specificity (100%) but a low sensitivity (8%). It offers a number of advantages over existing laboratory methods for diagnosing trachoma in the field. It is a rapid, simple, and easily transferred test that has a high specificity. Although DFA cytology shares the low sensitivity of other laboratory methods of diagnosing trachoma, we believe its logistical advantages make it the laboratory test of choice.
Wilson MC, Millan-Velasco F, Tielsch JM, Taylor HR. Direct-Smear Fluorescent Antibody Cytology as a Field Diagnostic Tool for Trachoma. Arch Ophthalmol. 1986;104(5):688-690. doi:10.1001/archopht.1986.01050170078026