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Article
October 1987

The Rapid Detection of Acanthamoeba in Paraffin-Embedded Sections of Corneal Tissue With Calcofluor White

Author Affiliations

From the Department of Ophthalmology, University of Texas Health Science Center at Dallas, Southwestern Medical School.

Arch Ophthalmol. 1987;105(10):1366-1367. doi:10.1001/archopht.1987.01060100068028
Abstract

Acanthamoeba keratitis is a difficult diagnosis to make with routine stains and cultures. Gram's, Giemsa, and hematoxylin-eosin stains do not differentially stain Acanthamoeba, making the detection of organisms difficult. Trophozoite and cyst forms in paraffin-embedded corneal tissue sections can be rapidly and differentially stained with calcofluor white. Under the fluorescence microscope, the trophozoites are bright red-orange, and cyst cell walls fluoresce bright apple-green with red-orange cytoplasm. Retrospective identification can be made by destaining hematoxylin-eosin-stained sections. Digesting background corneal tissue with trypsin or collagenase and hyaluronidase solutions helps to more readily identify trophozoites.

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