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January 1988

Interphotoreceptor Retinoid-Binding Protein Levels in Subretinal Fluid From Rhegmatogenous Retinal Detachment and Retinopathy of Prematurity

Author Affiliations

From the Lions Eye Research Laboratories, LSU Eye Center, LSU Medical Center School of Medicine, New Orleans (Dr Newsome); the Laboratory of Retinal Cell and Molecular Biology, National Institutes of Health, Bethesda, Md (Drs Chader and Wiggert); The Wilmer Ophthalmological Institute, The Johns Hopkins Medical Institutions, Baltimore (Drs Yeo and Welch); and the Department of Ophthalmology, University of Tennessee Center for Health Sciences, Memphis (Drs Blacharski and Charles).

Arch Ophthalmol. 1988;106(1):106-110. doi:10.1001/archopht.1988.01060130112039

• Interphotoreceptor retinoid-binding protein (IRBP) has been detected qualitatively in subretinal fluid associated with rhegmatogenous retinal detachments. We have used a quantitative enzyme-linked immunosorbent assay to determine the IRBP concentrations in 24 fluid specimens collected at retinal reattachment surgery from 22 patients, and from 17 patients who underwent surgery for retinopathy of prematurity with traction detachment. We observed a wide range of concentrations (1 to 176 μg of IRBP per milligram of protein). Higher concentrations were found only in fluid from the more recent rhegmatogenous detachments. The lowest concentrations were detected among fluids drained from under rhegmatogenous detachments ranging from five days' to one year's duration. The IRBP concentration in subretinal fluid showed no correlation with visual outcome and may be influenced by IRBP turnover in the vitreous cavity. Most strikingly, none of the 17 samples from patients with retinopathy of prematurity contained detectable amounts of IRBP.