July 1989

Disinfection of Goldmann Tonometers Against Human Immunodeficiency Virus Type 1

Author Affiliations

From the Departments of Ophthalmology (Drs Pepose and Lee) and Pathology (Dr Pepose), Washington University School of Medicine, St Louis, Mo; the Department of Microbiology, Georgetown University School of Medicine, Washington, DC (Dr Linette); and the Department of Ophthalmology, Oregon Health Sciences University, Portland, Ore (Dr MacRae). Dr Pepose is a faculty fellow of the American College of Surgeons (1988-1990).

Arch Ophthalmol. 1989;107(7):983-985. doi:10.1001/archopht.1989.01070020045026

• Goldmann tonometer tips were inoculated with 5 × 105 IU of cell-free or cell-associated human immunodeficiency virus type 1 (lymphadenopathy virus type 1 isolate) or 104 plaque-forming units of herpes simplex virus type 1 (McKrae strain) or type 2 (Hicks strain). In an effort to mimic a "worst case" clinical scenario, each respective virus was allowed to air dry on the tonometer tip for 10 minutes. Inoculated tonometers were then (1) not treated, (2) wiped with a disposable (Kimwipe) tissue or sterile gauze; (3) wiped with sterile gauze soaked with 3% hydrogen peroxide; or (4) wiped with a 70% isopropyl alcohol swab. The hydrogen peroxide treatment and the alcohol wipes both completely disinfected the tonometer tips for human immunodeficiency virus type 1 and herpes simplex virus types 1 and 2, whereas wiping with a sterile gauze or tissue was not effective. Wiping the Goldmann tonometer tip with an isopropyl alcohol swab and then allowing the alcohol to evaporate provides a ready and efficient means of inactivating these three enveloped viruses.