• Acanthamoeba keratitis is a sight-threatening disease that is difficult to treat. The development of an animal model is necessary for many of the studies required to improve visual outcome in human patients. A rat model is proposed that is dependent on coinoculation of amebae and corynebacteria into the corneal stroma. The infective dose was determined for a virulent Acanthamoeba isolate and was used to screen 17 other isolates, including 7 from the human cornea. A total of 6 were infective in the rat cornea. The model should be useful for controlled in vivo studies of this intractable condition.
Badenoch PR, Johnson AM, Christy PE, Coster DJ. Pathogenicity of Acanthamoeba and a Corynebacterium in the Rat Cornea. Arch Ophthalmol. 1990;108(1):107-112. doi:10.1001/archopht.1990.01070030113040