To the Editor.
—Vitreous specimens in endophthalmitis can be obtained by aspiration through a needle, vitrectomy biopsy procedure, or full vitrectomy approach. In theory, a vitreous biopsy procedure in which the vitreous is cut may be safer than vitreous aspiration. When performing a vitreous biopsy in endophthalmitis, it is widely believed1 that an infusion line is necessary to prevent the globe from collapsing. However, one can use a single 20-gauge sclerotomy without infusion to rapidly and simply perform a vitrectomy biopsy in this disease. Additional sclerotomy openings are not required. The suction outflow end of the vitrectomy cutter is connected to a "vitreous biopsy tubing adaptor," which consists of 2.5 cm of silicone tubing connected to a female Luer lock as shown in the Figure. The Luer lock is attached to a tuberculin syringe so that suction can be controlled manually. The cutting function is machine controlled.Under the
Doft BH, Donnelly K. A Single Sclerotomy Vitreous Biopsy Technique in Endophthalmitis. Arch Ophthalmol. 1991;109(4):465. doi:10.1001/archopht.1991.01080040025008