• A prospective, randomized, masked, placebo-controlled study was performed to determine whether a single, intraoperative subconjunctival application of a 0.5-mg/mL solution of mitomycin-C enhances the success of full-thickness filtration surgery in rabbits. Compared with control eyes, mitomycin-C-treated eyes showed significant increases in bleb duration from 8.1±2.4 to 68.0±20.8 days and in intraocular pressure reduction from 6.0±3.0 to 63.6±21.5 days. Histopathologic evaluation confirmed the inhibitory effects of mitomycin-C on fibrovascular, fibrocellular, and collagenous organization of the filtering blebs that resulted in their preservation. Transient, superficial, corneal vascularization anterior to the bleb occurred in all mitomycin-C-treated eyes. No other clinical or pathologic signs of undesirable side effects were noted. We studied the effectiveness and safety of a single intraoperative application of mitomycin-C in prolonging the success of filtration surgery in rabbits and its potential for similar use in humans.
Bergstrom TJ, Wilkinson WS, Skuta GL, Watnick RL, Elner VM. The Effects of Subconjunctival Mitomycin-C on Glaucoma Filtration Surgery in Rabbits. Arch Ophthalmol. 1991;109(12):1725-1730. doi:10.1001/archopht.1991.01080120109038