March 1992

An Ocular Melanoma-Associated AntigenMolecular Characterization

Author Affiliations

From the Retina Service, Wills Eye Hospital and Thomas Jefferson University (Drs Wang, Earley, and Donoso), and the Oncology Service, Wills Eye Hospital (Dr Shields), Philadelphia, Pa. Dr Wang is the Robert D. McCallum Research Scholar, and Dr Donoso is the Thomas D. Duane Clinical and Research Professor of Ophthalmology, Wills Eye Hospital.

Arch Ophthalmol. 1992;110(3):399-404. doi:10.1001/archopht.1992.01080150097036

• Monoclonal antibody (MAb) 8-1H defines a highly conserved ocular melanoma-associated antigen. Based on a potential binding site of MAb8-1, we identified, molecularly cloned, and sequenced the gene, encoding this melanoma-associated antigen from a uveal melanoma copy DNA library. Nucleotide sequence analysis of the melanoma-associated antigen revealed characteristics of a membrane-bound protein of 238 amino acids (approximately 25 kd) with four transmembrane domains. The sequence of the ocular melanoma-associated antigen is identical to the sequence of a recently reported cutaneous melanomaassociated antigen, substantiating our previous studies regarding common antigens between ocular and cutaneous melanoma. In addition, the melanomaassociated antigen shares sequence homology with a number of transmembrane proteins that appear to be involved in growth regulation. The implication of the biological function of this ocular melanoma-associated antigen, its relevance to the malignant phenotype, and clinical applications of the molecular characterization of this melanoma-associated antigen are described.