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September 1994

Fresh-Frozen Cucumber as a Mount for Conjunctival and Corneal Tissue in Cryomicrotomy

Author Affiliations

Nottingham, England
Philadelphia, Pa

Arch Ophthalmol. 1994;112(9):1139-1141. doi:10.1001/archopht.1994.01090210023005

Conjunctival tissue is very elastic and has a strong tendency to roll or fold on itself, making proper histological orientation fairly difficult. This interferes with accurate histological diagnosis because normal anatomical relationships are often distorted, and tangentially or obliquely cut tissue can introduce errors in interpretation.1,2 Mounting conjunctiva on "cucumber paper" prepared by desiccating cucumber in alcohol obviates many of these problems for formalin-fixed paraffin sections.1,2 However, a suitable mount for obtaining frozen sections of ocular surface tissue has not been described. Frozen sections allow for immediate histological diagnosis. Furthermore, epitopes for many antibody markers are lost during the process of chemical fixation, necessitating the use of frozen sections. We successfully used cucumber as a mount for cryomicrotomy of normal and pathological conjunctival and corneal tissue.

Methods.  Small blocks of fresh or fresh-frozen peeled cucumber, cleaned of all its seeds, were prepared. Attempts to store cucumber pieces in

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