[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.161.130.145. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Download PDF
Figure 1. Clinical photograph and photomicrographs. A, Right conjunctival mass, with a yellow gelatinous temporal lesion extending from the 9-o’clock position to the 12-o’clock position with associated symblepharon and feeder vessels. B, Invasive squamous cell carcinoma, moderately differentiated (hematoxylin-eosin, original magnification ×400). C, Conjunctival squamous cell carcinoma and Leishmania amastigotes in histiocytes (hematoxylin-eosin, original magnification ×1000). D, CD68-positive histiocytes containing organisms (immunoperoxidase reaction, original magnification ×1000).

Figure 1. Clinical photograph and photomicrographs. A, Right conjunctival mass, with a yellow gelatinous temporal lesion extending from the 9-o’clock position to the 12-o’clock position with associated symblepharon and feeder vessels. B, Invasive squamous cell carcinoma, moderately differentiated (hematoxylin-eosin, original magnification ×400). C, Conjunctival squamous cell carcinoma and Leishmania amastigotes in histiocytes (hematoxylin-eosin, original magnification ×1000). D, CD68-positive histiocytes containing organisms (immunoperoxidase reaction, original magnification ×1000).

Figure 2. Axial abdominal computed tomography scan exhibiting hepatosplenomegaly, with the liver and spleen measuring 26.5 cm and 17 cm, respectively (largest axial dimension).

Figure 2. Axial abdominal computed tomography scan exhibiting hepatosplenomegaly, with the liver and spleen measuring 26.5 cm and 17 cm, respectively (largest axial dimension).

1.
Maclean H, Dhillon B, Ironside J. Squamous cell carcinoma of the eyelid and the acquired immunodeficiency syndrome.  Am J Ophthalmol. 1996;121(2):219-221PubMed
2.
Lewallen S, Shroyer KR, Keyser RB, Liomba G. Aggressive conjunctival squamous cell carcinoma in three young Africans.  Arch Ophthalmol. 1996;114(2):215-218PubMedArticle
3.
Tulvatana W, Tirakunwichcha S. Multifocal squamous cell carcinoma of the conjunctiva with intraocular penetration in a patient with AIDS.  Cornea. 2006;25(6):745-747PubMedArticle
4.
Suster S, Ronnen M. Basal cell carcinoma arising in a Leishmania scar.  Int J Dermatol. 1988;27(3):175-176PubMedArticle
5.
Matayoshi S, Baddina-Caramelli C, Goldbaum M, Takei LM, Honda M, Kara-José N. Epidermoid carcinoma arising in an ocular Leishmania lesion.  Br J Ophthalmol. 2000;84(11):1331-1332PubMedArticle
6.
Auyeung P, French MA, Hollingsworth PN. Immune restoration disease associated with Leishmania donovani infection following antiretroviral therapy for HIV infection.  J Microbiol Immunol Infect. 2010;43(1):74-76PubMedArticle
7.
Bernier R, Barbeau B, Tremblay MJ, Olivier M. The lipophosphoglycan of Leishmania donovani up-regulates HIV-1 transcription in T cells through the nuclear factor-kappaB elements.  J Immunol. 1998;160(6):2881-2888PubMed
8.
Wolday D, Berhe N, Britton S, Akuffo H. HIV-1 alters T helper cytokines, interleukin-12 and interleukin-18 responses to the protozoan parasite Leishmania donovani AIDS. 2000;14(8):921-929PubMedArticle
9.
el Hassan AM, Khalil EA, el Sheikh EA, Zijlstra EE, Osman A, Ibrahim ME. Post kala-azar ocular leishmaniasis.  Trans R Soc Trop Med Hyg. 1998;92(2):177-179PubMedArticle
10.
el-Hassan AM, el-Sheikh EA, Eltoum IA,  et al.  Post-kala-azar anterior uveitis: demonstration of Leishmania parasites in the lesion.  Trans R Soc Trop Med Hyg. 1991;85(4):471-473PubMedArticle
11.
Montero JA, Ruiz-Moreno JM, Sanchis E. Intraretinal hemorrhage associated with leishmaniasis.  Ophthalmic Surg Lasers Imaging. 2003;34(3):212-214PubMed
12.
Huna-Baron R, Warren FA, Miller W, Jacobs J, Green J, Kupersmith MJ. Mucosal leishmaniasis presenting as sinusitis and optic neuropathy.  Arch Ophthalmol. 2000;118(6):852-854PubMed
Research Letters
Sep 2011

Conjunctival Squamous Cell Carcinoma Harboring Leishmania Amastigotes in a Human Immunodeficiency Virus–Positive Patient

Author Affiliations

Author Affiliations: Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, Florida (Dr Bielory); and Departments of Ophthalmology (Drs Lari and Turbin), Pathology and Laboratory Medicine (Drs Mirani and Fitzhugh), and Medicine (Dr Kapila), New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark.

Arch Ophthalmol. 2011;129(9):1230-1231. doi:10.1001/archophthalmol.2011.252

Leishmaniasis, a protozoal infection transmitted via the sand fly bite, is endemic to India, the Middle East, and Africa and is periodically found in Central and South America. Visceral leishmaniasis, also known as kala-azar, black fever, or Dumdum fever, is the most severe form. Ocular involvement occurs more frequently in cutaneous than in mucocutaneous and visceral manifestations. We report a unique case of Leishmania donovani chagasi identified by biopsy of squamous cell carcinoma (SCC) of the bulbar conjunctiva in a human immunodeficiency virus (HIV)–positive Hispanic man. Subsequent evaluation revealed kala-azar with histopathological confirmation of the organism in conjunctiva, lacrimal gland, and liver specimens.

Report of a Case

A 39-year-old HIV-positive Guatemalan man had decreased vision, epiphora, and pain in the right eye for 18 months. He had been continuously maintained on highly active antiretroviral therapy, azithromycin, and sulfamethoxazole/trimethoprim for 2 years. He denied fever, sweating, or flulike symptoms.

Best-corrected visual acuity was 20/25 OD and 20/20 OS. The pupils were 5 mm on the right and 7 mm on the left, briskly reactive to light, and without relative afferent pupil defect. Extraocular movements were full without restriction. Intraocular pressures were 17 mm Hg in both eyes. The right upper eyelid was mildly ptotic and swollen. The slitlamp biomicroscopic appearance is shown in Figure 1A. Funduscopic examination results were unremarkable. Ultrasound biomicroscopy and B-mode ultrasonography of the globe did not suggest extension into deeper structures or transscleral invasion.

Computed tomography of the orbit revealed disease limited to preseptal soft tissue. Laboratory evaluation demonstrated a viral load of less than 48 copies/mL and a CD4 lymphocyte count of 79 cells/μL. Excisional biopsy of the mass showed moderately differentiated invasive SCC as well as intracellular microorganisms in histiocytes (Figure 1B). Special stains for Histoplasma and Toxoplasma were negative. High-power oil immersion highlighted the Leishmania amastigotes (Figure 1C), and CD68 staining confirmed their presence in macrophages (Figure 1D). The Centers for Disease Control and Prevention confirmed the microorganisms as L donovani chagasi.

Postoperative positron emission tomography showed increased uptake within the right lateral conjunctiva, liver, spleen, and axillary, mediastinal, mesenteric, pelvic, and cervical lymph nodes. Abdominal computed tomography revealed hepatosplenomegaly (Figure 2). A subsequent liver biopsy confirmed the diagnosis of visceral leishmaniasis. The patient was treated with intravenous liposomal amphotericin B (190 mg/d for 14 days and triweekly thereafter).

Comment

In HIV-infected individuals, SCC occurs at an earlier age and may be more aggressive than in immunocompetent individuals. The oropharynx, cervix, and anorectum are most frequently involved, but the disease can manifest in the eyelids,1 conjunctiva,2 and iris.3 Rare cases of Leishmania infection within basal cell carcinoma4 and SCC5 have been reported, including a single Brazilian case of mucocutaneous leishmaniasis of the conjunctiva with an ensuing epidermoid carcinoma in the orbit.5 To our knowledge, we report the first case of visceral leishmaniasis within conjunctival SCC.

Visceral leishmaniasis commonly affects the liver and spleen. There is a strong association between HIV and visceral leishmaniasis, with an increasing incidence in nonendemic regions.6 Exploiting the host immune system, Leishmania upregulates HIV via nuclear factor κ B, interferon γ,7 interleukin 12, and/or interleukin 18.8 There are reports of the ocular manifestations of Leishmania including blepharoconjunctivitis,9,10 anterior uveitis,9,10 thrombocytopenic intraretinal hemorrhages,11 and optic neuropathy12 from an orbital apex lesion. Pentavalent antimonial compounds such as sodium stibogluconate and meglumine antimoniate have been the traditional pharmacologic intervention. However, polyene antifungals such as amphotericin B or the antiprotozoal medication miltefosine are the current standards of care. Plans for additional treatment of the SCC will be directed by the patient's response to antimicrobial therapy.

Back to top
Article Information

Correspondence: Dr Turbin, Institute of Ophthalmology and Visual Science, 90 Bergen St, DOC Bldg, Ste 6177, Newark, NJ 07103 (turbinre@umdnj.edu).

Financial Disclosure: None reported.

Funding/Support: This work was supported in part by an unrestricted grant from Research to Prevent Blindness, New York, New York, The Eye Institute of New Jersey, Newark, and the Gene C. Coppa Memorial Fund, Newark.

Role of the Sponsors: The sponsors have provided general academic support for Dr Turbin's academic work but have had no role in collection, analysis, interpretation, preparation, or review.

References
1.
Maclean H, Dhillon B, Ironside J. Squamous cell carcinoma of the eyelid and the acquired immunodeficiency syndrome.  Am J Ophthalmol. 1996;121(2):219-221PubMed
2.
Lewallen S, Shroyer KR, Keyser RB, Liomba G. Aggressive conjunctival squamous cell carcinoma in three young Africans.  Arch Ophthalmol. 1996;114(2):215-218PubMedArticle
3.
Tulvatana W, Tirakunwichcha S. Multifocal squamous cell carcinoma of the conjunctiva with intraocular penetration in a patient with AIDS.  Cornea. 2006;25(6):745-747PubMedArticle
4.
Suster S, Ronnen M. Basal cell carcinoma arising in a Leishmania scar.  Int J Dermatol. 1988;27(3):175-176PubMedArticle
5.
Matayoshi S, Baddina-Caramelli C, Goldbaum M, Takei LM, Honda M, Kara-José N. Epidermoid carcinoma arising in an ocular Leishmania lesion.  Br J Ophthalmol. 2000;84(11):1331-1332PubMedArticle
6.
Auyeung P, French MA, Hollingsworth PN. Immune restoration disease associated with Leishmania donovani infection following antiretroviral therapy for HIV infection.  J Microbiol Immunol Infect. 2010;43(1):74-76PubMedArticle
7.
Bernier R, Barbeau B, Tremblay MJ, Olivier M. The lipophosphoglycan of Leishmania donovani up-regulates HIV-1 transcription in T cells through the nuclear factor-kappaB elements.  J Immunol. 1998;160(6):2881-2888PubMed
8.
Wolday D, Berhe N, Britton S, Akuffo H. HIV-1 alters T helper cytokines, interleukin-12 and interleukin-18 responses to the protozoan parasite Leishmania donovani AIDS. 2000;14(8):921-929PubMedArticle
9.
el Hassan AM, Khalil EA, el Sheikh EA, Zijlstra EE, Osman A, Ibrahim ME. Post kala-azar ocular leishmaniasis.  Trans R Soc Trop Med Hyg. 1998;92(2):177-179PubMedArticle
10.
el-Hassan AM, el-Sheikh EA, Eltoum IA,  et al.  Post-kala-azar anterior uveitis: demonstration of Leishmania parasites in the lesion.  Trans R Soc Trop Med Hyg. 1991;85(4):471-473PubMedArticle
11.
Montero JA, Ruiz-Moreno JM, Sanchis E. Intraretinal hemorrhage associated with leishmaniasis.  Ophthalmic Surg Lasers Imaging. 2003;34(3):212-214PubMed
12.
Huna-Baron R, Warren FA, Miller W, Jacobs J, Green J, Kupersmith MJ. Mucosal leishmaniasis presenting as sinusitis and optic neuropathy.  Arch Ophthalmol. 2000;118(6):852-854PubMed
×