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Figure 1. Magnetic resonance imaging, clinical, and biopsy findings. A, Coronal projection of a magnetic resonance imaging scan reveals a large area of central lucency (arrow) in an enlarged lacrimal gland, consistent with extensive necrosis or a sterile abscess. Inset, Swollen, erythematous right upper eyelid with prolapse of the chemotic conjunctiva. B, Swelling is present bilaterally and more posteriorly in the orbit's lateral rectus muscle (arrows) and inferior rectus muscle (arrowheads). The optic nerves (ON) are not compressed. C, Focal hemorrhagic necrosis in the periorbita (hematoxylin-eosin, original magnification ×100). Inset, Large nuclei with a ground-glass characteristic (arrows) suggestive of viral infection (hematoxylin-eosin, original magnification ×400). D, Abundant inflammatory cells are present between the acini (AC) of the lacrimal gland (hematoxylin-eosin, original magnification ×100). Inset, Focus of granular necrosis of the lacrimal parenchyma (hematoxylin-eosin, original magnification ×100).

Figure 1. Magnetic resonance imaging, clinical, and biopsy findings. A, Coronal projection of a magnetic resonance imaging scan reveals a large area of central lucency (arrow) in an enlarged lacrimal gland, consistent with extensive necrosis or a sterile abscess. Inset, Swollen, erythematous right upper eyelid with prolapse of the chemotic conjunctiva. B, Swelling is present bilaterally and more posteriorly in the orbit's lateral rectus muscle (arrows) and inferior rectus muscle (arrowheads). The optic nerves (ON) are not compressed. C, Focal hemorrhagic necrosis in the periorbita (hematoxylin-eosin, original magnification ×100). Inset, Large nuclei with a ground-glass characteristic (arrows) suggestive of viral infection (hematoxylin-eosin, original magnification ×400). D, Abundant inflammatory cells are present between the acini (AC) of the lacrimal gland (hematoxylin-eosin, original magnification ×100). Inset, Focus of granular necrosis of the lacrimal parenchyma (hematoxylin-eosin, original magnification ×100).

Figure 2. Histopathologic findings. A, In the lacrimal gland, the CD3-positive T-lymphocytic population is intense (immunoperoxidase reaction, toluidine blue with diaminobenzidine chromogen, original magnification ×200). B, Plentiful Epstein-Barr virus–positive T lymphocytes are located between the acini (AC) of the lacrimal gland parenchyma (in situ hybridization with Epstein-Barr virus probe, original magnification ×100). ID indicates intralobular duct. C, The lung biopsy manifests a lymphocytic infiltrate with striking perivascular cuffing toward the bottom right (hematoxylin-eosin, original magnification ×25). Inset, The cells are shown to be CD3-positive T lymphocytes (immunoperoxidase reaction, toluidine blue with diaminobenzidine chromogen, original magnification ×100). D, Epstein-Barr virus–positive lymphocytes are responsible for the perivascular lung infiltrate (in situ hybridization with Epstein-Barr virus probe, original magnification ×100).

Figure 2. Histopathologic findings. A, In the lacrimal gland, the CD3-positive T-lymphocytic population is intense (immunoperoxidase reaction, toluidine blue with diaminobenzidine chromogen, original magnification ×200). B, Plentiful Epstein-Barr virus–positive T lymphocytes are located between the acini (AC) of the lacrimal gland parenchyma (in situ hybridization with Epstein-Barr virus probe, original magnification ×100). ID indicates intralobular duct. C, The lung biopsy manifests a lymphocytic infiltrate with striking perivascular cuffing toward the bottom right (hematoxylin-eosin, original magnification ×25). Inset, The cells are shown to be CD3-positive T lymphocytes (immunoperoxidase reaction, toluidine blue with diaminobenzidine chromogen, original magnification ×100). D, Epstein-Barr virus–positive lymphocytes are responsible for the perivascular lung infiltrate (in situ hybridization with Epstein-Barr virus probe, original magnification ×100).

1.
Jakobiec FA. Ocular adnexal lymphoid tumors: progress in need of clarification.  Am J Ophthalmol. 2008;145(6):941-950PubMedArticle
2.
Woog JJ, Kim YD, Yeatts RP,  et al.  Natural killer/T-cell lymphoma with ocular and adnexal involvement.  Ophthalmology. 2006;113(1):140-147PubMedArticle
3.
Wieczorek R, Jakobiec FA, Sacks EH, Knowles DM. The immunoarchitecture of the normal human lacrimal gland: relevancy for understanding pathologic conditions.  Ophthalmology. 1988;95(1):100-109PubMed
4.
Jaffe ES, Pittaluga S. Lymphomatoid granulomatosis. In: Jaffe E, Harris N, Vardiman J, Campo E, Arber D, eds. Hematopathology. St Louis, MO: Elsevier/Saunders; 2011:382-390
5.
Quintanilla-Martinez L, Kimura H, Jaffe ES. EBV-positive T-cell lymphoproliferative disorders of childhood. In: Swerdlow SH, Campo E, Harris NL, eds. Classification of Tumors of the Haematopoietic and Lymphoid Tissues. Lyon, France: International Agency for Research on Cancer; 2008:278-280
6.
Ko YH, Jaffe ES. Epstein-Barr virus-positive systemic lymphoproliferative disorders and related lymphoproliferations of childhood. In: Jaffe ES, Harris NL, Vardiman JW, Campo E, Arber DA, eds. Hematopathology. St Louis, MO: Elsevier/Saunders; 2011:492-505
7.
Park S, Kim K, Kim WS, Yoo KH, Koo HH, Ko YH. Systemic EBV+ T-cell lymphoma in elderly patients: comparison with children and young adult patients.  Virchows Arch. 2008;453(2):155-163PubMedArticle
8.
Reifler DM, Warzynski MJ, Blount WR, Graham DM, Mills KA. Orbital lymphoma associated with acquired immune deficiency syndrome (AIDS).  Surv Ophthalmol. 1994;38(4):371-380PubMedArticle
9.
Douglas RS, Goldstein SM, Katowitz JA,  et al.  Orbital presentation of posttransplantation lymphoproliferative disorder: a small case series.  Ophthalmology. 2002;109(12):2351-2355PubMedArticle
10.
Papalkar D, Sharma S, Francis IC, Downie JA, Thanakrishnan G, Hughes LJ. A rapidly fatal case of T-cell lymphoma presenting as idiopathic orbital inflammation.  Orbit. 2005;24(2):131-133PubMedArticle
Research Letters
Apr 2012

Epstein-Barr Virus–Positive T-Cell Lymphoma Involving the Lacrimal Gland of an Adult

Author Affiliations

Author Affiliations: David G. Cogan Laboratory of Ophthalmic Pathology and Division of Oculoplastic Surgery, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.

Arch Ophthalmol. 2012;130(4):523-525. doi:10.1001/archophthalmol.2011.1728

Systemic T-cell lymphomas metastatic to the orbit are much rarer than non-Hodgkin B-cell neoplasms (predominantly marginal zone and follicular).1 We describe an adult with an Epstein-Barr virus (EBV)–positive T-cell lymphoma of the lacrimal gland associated with multiorgan disease that was not of the expected natural killer/T-cell subtype.2 Because of the unusual clinical findings and imaging study results, the diagnosis was elusive. A lacrimal biopsy evaluated with an EBV probe established the correct diagnosis; this technique should probably be used for all unusual or atypical orbital lymphomas.

Report of a Case

A 57-year-old man developed abrupt right eye swelling and erythema with chemosis that worsened over 6 days and was nonresponsive to intravenous antibiotics (Figure 1A). He had had a febrile illness with fatigue for 4 years. Earlier lung, liver, and bone marrow biopsies revealed an EBV-positive T-cell lymphoma with a clonal rearrangement of the T-cell receptor gene. Visual acuity was 20/20 OU. The motility was moderately decreased and there was no proptosis. Magnetic resonance imaging showed enlargement of the lacrimal gland on the right side with a nonenhancing center (Figure 1A) and bilateral involvement of the extraocular muscles (Figure 1B). Biopsy of the periorbita (Figure 1C) and lacrimal gland (Figure 1D) showed chronic inflammation with necrosis (Figure 1C and D) and scattered larger cells with ground-glass nuclei (Figure 1C). The lymphocytes were positive for CD3 and CD5 (Figure 2A) and negative for CD56; there were rare CD20-positive cells. Brown-Hopps, Steiner, and Gomori methenamine silver stains disclosed no organisms. In situ hybridization with an EBV probe demonstrated marked positivity in the lymphocytes in both the periorbita and lacrimal gland (Figure 2B). The lung biopsy showed a striking perivascular lymphocytic distribution (Figure 2C) with identical immunohistochemical and EBV-positive (Figure 2D) findings. Radiotherapy delivered to both orbits caused complete resolution of the patient's symptoms, and he has recently received an allogenic bone marrow transplant.

Comment

Ascending ductular and hematogenous infections of the lacrimal gland are vanishingly rare. Our patient's orbital “cellulitis” with lacrimal gland cavitation and no response to antibiotic therapy was confusing. The biopsy revealed necrosis of the lacrimal gland (probably at the margins of a sterile abscess) and the profuse presence of CD3- and CD5-positive T lymphocytes that were CD56 negative, thereby ruling out a natural killer/T-cell lymphoma. The number of T cells was much greater than that normally expected in the gland,3 and in situ hybridization established T-cell EBV positivity.

The liver biopsy had disclosed that the lymphocytic infiltrate showed a monoclonal T-cell receptor gene rearrangement, rendering the condition a true lymphoma rather than a lymphoproliferative disorder. Angiodestruction and necrosis frequently accompany EBV-induced lymphoproliferations by means of locally released chemokines4; an open lung biopsy displayed a prominent tendency for vascular cuffing by EBV-positive T cells. The multisystem involvement of lung, liver, and bone marrow and the favorable response to bilateral orbital radiotherapy support the neoplastic character of the condition.

The closest analog to the current lesion is the T-cell lymphoproliferative disorder of childhood without a known clonal rearrangement.5,6 To our knowledge, our case is the first orbital example of an EBV-positive monoclonal T-cell lymphoma not of the natural killer/T-cell subtype in an adult.7 The EBV-positive lymphomas of the orbit have mostly, but not exclusively, been B-cell neoplasms such as Burkitt lymphoma, lymphomatoid granulomatosis, and AIDS-associated and posttransplantation lymphomas.4,8,9 Orbital natural killer/T-cell lymphoma (CD3, CD5, and CD56 positive) with EBV infection can also cause a clinical inflammatory picture.10

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Article Information

Correspondence: Dr Jakobiec, David G. Cogan Laboratory of Ophthalmic Pathology, Massachusetts Eye and Ear Infirmary, 243 Charles St, Room 321, Boston, MA 02114 (fred_jakobiec@meei.harvard.edu).

Financial Disclosure: None reported.

References
1.
Jakobiec FA. Ocular adnexal lymphoid tumors: progress in need of clarification.  Am J Ophthalmol. 2008;145(6):941-950PubMedArticle
2.
Woog JJ, Kim YD, Yeatts RP,  et al.  Natural killer/T-cell lymphoma with ocular and adnexal involvement.  Ophthalmology. 2006;113(1):140-147PubMedArticle
3.
Wieczorek R, Jakobiec FA, Sacks EH, Knowles DM. The immunoarchitecture of the normal human lacrimal gland: relevancy for understanding pathologic conditions.  Ophthalmology. 1988;95(1):100-109PubMed
4.
Jaffe ES, Pittaluga S. Lymphomatoid granulomatosis. In: Jaffe E, Harris N, Vardiman J, Campo E, Arber D, eds. Hematopathology. St Louis, MO: Elsevier/Saunders; 2011:382-390
5.
Quintanilla-Martinez L, Kimura H, Jaffe ES. EBV-positive T-cell lymphoproliferative disorders of childhood. In: Swerdlow SH, Campo E, Harris NL, eds. Classification of Tumors of the Haematopoietic and Lymphoid Tissues. Lyon, France: International Agency for Research on Cancer; 2008:278-280
6.
Ko YH, Jaffe ES. Epstein-Barr virus-positive systemic lymphoproliferative disorders and related lymphoproliferations of childhood. In: Jaffe ES, Harris NL, Vardiman JW, Campo E, Arber DA, eds. Hematopathology. St Louis, MO: Elsevier/Saunders; 2011:492-505
7.
Park S, Kim K, Kim WS, Yoo KH, Koo HH, Ko YH. Systemic EBV+ T-cell lymphoma in elderly patients: comparison with children and young adult patients.  Virchows Arch. 2008;453(2):155-163PubMedArticle
8.
Reifler DM, Warzynski MJ, Blount WR, Graham DM, Mills KA. Orbital lymphoma associated with acquired immune deficiency syndrome (AIDS).  Surv Ophthalmol. 1994;38(4):371-380PubMedArticle
9.
Douglas RS, Goldstein SM, Katowitz JA,  et al.  Orbital presentation of posttransplantation lymphoproliferative disorder: a small case series.  Ophthalmology. 2002;109(12):2351-2355PubMedArticle
10.
Papalkar D, Sharma S, Francis IC, Downie JA, Thanakrishnan G, Hughes LJ. A rapidly fatal case of T-cell lymphoma presenting as idiopathic orbital inflammation.  Orbit. 2005;24(2):131-133PubMedArticle
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