Choroideremia (Mendelian Inheritance in Man [MIM] #303100) is an uncommon X-linked chorioretinal degeneration characterized by mottling of the retinal pigment epithelium (RPE) and inexorable centripetal extension of areas of choroidal and outer retinal atrophy. Extensive rod and cone system dysfunction characteristically occurs within the first years of life. Eventually, the rod electroretinogram (ERG) becomes undetectable and cone responses are recordable only with computer averaging.1 Nyctalopia and progressive field constriction typically become evident in the second decade with acuity loss eventually occurring because of macula involvement. Female carriers often exhibit “moth-eaten,” patchy pigmentation at the level of the RPE compatible with lyonization.2
We describe the finding of normal or mildly subnormal ERGs in 3 young affected males (aged 8, 9, and 10 years) from 2 unrelated families with choroideremia (Figure 1). Direct sequencing of the choroideremia gene (CHM) identified a different nonsense (stop) mutation in each family. The mutations were only 14 codons apart in exon 6, suggesting that a common mechanism underlying these mutations may lead to a milder clinical and electrophysiological phenotype.
Pedigrees for case 1 (A) and cases 2 and 3 (B). Black squares indicate affected males; circles with black dots, known carrier females; gray squares, males affected by history.
The patient representing case 1 was asymptomatic when evaluated at age 6 years. Visual acuity was 20/20-2 OD and 20/20 OS. Fundus examination disclosed fine, peppery RPE mottling in the midperiphery but normal-appearing optic discs, retinal vasculature, and maculae (Figure 2A-C). Bone spicule formation and choroidal atrophy were absent. His maternal grandfather had advanced choroideremia (Figure 2D).
Color fundus photographs of affected individuals from the affected families. A-C, Case 1. The right eye, left eye, and peripheral view of the left eye at ages 6, 10, and 12 years, respectively. D, His maternal grandfather at age 71 years. E and F, Case 2. The right eye and left eye, respectively.
Electroretinograms (Figure 3A) performed to standards from the International Society for Clinical Electrophysiology of Vision demonstrated, at age 10 years, mildly subnormal responses of rods (67% of normal mean; lower limit, 83% of mean) and, to a greater degree, dark-adapted cones (44% of normal mean; normal lower limit, 76%) with normal amplitudes of light-adapted cones consistent with mild rod and scotopic cone dysfunction. Rod and cone implicit times were normal. Repeat ERGs at 12 years of age showed minimal further rod loss. Karyotyping was normal. Direct sequencing of CHM identified a nonsense mutation (R239X, CGA→CTA), also identified in the patient’s maternal grandfather.2,3
Electroretinograms (ERGs) of affected individuals performed to International Society for Clinical Electrophysiology of Vision standards. A, Case 1. The normal ERG is a composite average of 22 controls who were 7 to 20 years of age. For case 1, the right and left eyes are superimposed. Testing included, in addition, a red stimulus that was balanced to give equal rod responses to the blue stimulus. This stimulus produced greater loss of the early dark-adapted cone responses (arrows) than later-occurring rod responses. B, Case 2 demonstrated normal cone and rod amplitudes and implicit times. Case 3 demonstrated normal cone responses but notably reduced rod responses. The left eye tracings are shown. The vertical scale is 100 μV per division. The error bars for the B waves show the 95% confidence ranges for normals. OPs indicates oscillatory potentials.
The patient representing case 2, who is from an unrelated family with choroideremia, was first evaluated at 9 years of age. He was asymptomatic with visual acuities of 20/20 OU. The results of color vision testing and Goldmann perimetry testing were normal. Fundus examination identified fine, peppery RPE mottling in the midperiphery but normal-appearing optic discs, retinal vasculature, and maculae (Figure 2E and F). Bone spicule–like pigment formations and choroidal atrophy were not observed. The ERG responses were normal (Figure 3B); responses from a repeat ERG at age 11 years remained normal.
The patient representing case 3 is the maternal cousin of the patient in case 2 and was also asymptomatic when evaluated at 8 years of age. Visual acuity was 20/25 OD and 20/20-1 OS. Color vision screening was normal. Goldmann perimetry showed mild superotemporal restriction to the II-2e test target. Fundus examination revealed diffuse mottling of the RPE throughout the posterior pole and midperipheral retina similar to the moth-eaten appearance of choroideremia carriers. Electroretinogram testing (Figure 3B) showed normal cone amplitudes and implicit times to single flash and 30-Hz flicker. Rod amplitudes were reduced approximately 40%. The maximal dark-adapted B-wave response was reduced approximately 50%. Direct sequencing of CHM in cases 2 and 3 identified a nonsense mutation (R253X, CGA→TGA).2,3
Choroideremia, like other X-linked retinal disorders, shows variability in the degree to which hemizygous individuals are affected. Typically, disparities are greater between families than among members of the same family. For example, among Danish patients with various mutations in the CHM gene, Rosenberg and Schwartz4 found a 25-year age difference between individuals in the age at which time the peripheral visual field became unquantifiable by the Esterman method. Variation has also been reported in patients with choroideremia with the same mutation.5
Our patients are notable not only for their mild clinical phenotype but also for the mild nature of ERG abnormalities. The ERG in choroideremia has been studied by many investigators and is almost uniformly severely abnormal early in the course of the disease.1 Previously, Ponjavic et al5 reported mildly subnormal ERG responses in a 6-year-old boy in a family with choroideremia. Other affected members within this branch of the family had ERG responses that were more intact than those of more distant relatives, suggesting that factors other than the mutation itself may be determinants of severity and clinical course.5 Our observations highlight that the absence of markedly abnormal ERG responses at an early age does not necessarily exclude the diagnosis of choroideremia and serves to emphasize the need for follow-up testing in these patients.
The CHM gene encodes REP-1 (Rab escort protein-1), a ubiquitously expressed “housekeeping” protein. The protein REP-1 is essential for the regulation (isoprenylation) of Rab GTPases, which are then able to bind the cytoplasmic face of intracellular organelles. Rab GTPases appear to function as molecular switches activating “effector” proteins that guide intracellular vesicular transport.6 Mutations in REP-1 are not lethal because a related gene product, REP-2, serves the needs of all tissues except those of the eye.7 Although the precise mechanism by which chorioretinal degeneration occurs remains to be elucidated, histological analyses of choroideremia patients point toward the rod photoreceptors or RPE as the initial sites of pathologic abnormalities.8 Failure of vesicular transport of, for example, the Rab GTPase Rab27a (which has a greater affinity for REP-1 than REP-2) may lead to abnormal photoreceptor opsin transport, RPE phagocytosis, and lysosomal degradation or melanin granule transport.9
Both mutations in our families lie in a segment of the gene encoding the second “sequence conserved region” of the REP-1 protein. X-ray crystallographic studies of the bovine ortholog propose that this region facilitates tight molecular folding, and sequence analysis suggests that this domain may also mediate protein × protein interactions.6 Although nonsense mutations usually result in unstable messenger RNA molecules that are rapidly degraded, there is evidence that when stop mutations are located toward the 3′ end of the message, protein translation can proceed.10 However, the mutations in our cases fall within the middle of the gene. The cause for the mild phenotype early in the course of disease in these individuals is unknown but may arise because of environmental influences, epigenetic factors, or genetic modifiers that allow the transcriptional apparatus to read through the premature stop codon, resulting in a translation of some functional protein, or from sequence variation in the REP-1–interacting Rab GTPases and their substrates that may compensate for the defective gene product. Our data contribute to the phenotypic spectrum that may be encountered in this disorder and identify 2 specific mutations in CHM that are associated with a milder phenotype within the first decade of life.
Correspondence: Dr Weleber, Casey Eye Institute, Oregon Health and Science University, 3375 SW Terwilliger Blvd, Portland, OR 97239-4197 (firstname.lastname@example.org).
Financial Disclosure: None.
Francis PJ, Fishman GA, Trzupek KM, MacDonald IM, Stone EM, Weleber RG. Stop Mutations in Exon 6 of the Choroideremia Gene, CHM, Associated With Preservation of the Electroretinogram. Arch Ophthalmol. 2005;123(8):1146-1149. doi:10.1001/archopht.123.8.1146