[Skip to Content]
[Skip to Content Landing]
August 1971

The Anterior Segments of Rabbits After Alkali BurnsMetabolic and Histologic Alterations

Author Affiliations

From the Department of Cornea Research, Retina Foundation, and the Cornea Service, Massachusetts Eye and Ear Infirmary, Boston.

Arch Ophthalmol. 1971;86(2):189-193. doi:10.1001/archopht.1971.01000010191013

Alkali-burned rabbit eyes were analyzed for stromal and aqueous glucose and lactate, corneal hydration, and histologic changes. Severe alkali burns depressed aqueous glucose levels to an average of 35% of normal in 24 hours and to 5% of normal in 7 and 14 days. The stromal glucose level of 4.2μmol/gm H2O was lowered to an average of 60% of normal in 24 hours and to 26% of normal in 7 and 14 days. Stromal and aqueous lactates did not change. Stromal hydration was increased 24 hours after burning but was normal 7 and 14 days after burning. Milder alkali burns of the anterior segment produced less alteration of aqueous and stromal glucose concentrations. Severe hypotony and reduced open vascular channels in the iris and ciliary body suggest inadequate aqueous production through insufficient circulation.

Hughes WF Jr:  Alkali burns of the eye: II. Clinical and pathological course . Arch Ophthal 36:189-214, 1946.Article
Ballen PH:  Mucous membrane grafts in chemical (lye) burns . Amer J Ophthal 55:302-312, 1963.
Slansky HH, Freeman MI, Itoi M:  Collagenolytic activity in bovine corneal epithelium . Arch Ophthal 80:496-498, 1968.Article
Brown SI, Wassermann HE, Dunn MWL:  Alkali burns of the cornea . Arch Ophthal 82:91-94, 1969.Article
Slansky HH, Gnädinger MC, Itoi M, et al:  Collagenase in corneal ulcerations . Arch Ophthal 82:108-111, 1969.Article
Gnädinger MC, Itoi M, Slansky HH, et al:  The role of collagenase in the alkali-burned cornea . Amer J Ophthal 68:478-483, 1969.
Hughes WF Jr:  Alkali burns of the eye: I. Review of the literature and summary of present knowledge . Arch Ophthal 35:423-449, 1946.Article
Pichler A:  Ueber Ammoniak-Verätzung des Auges und der äusseren Haut . Z Augenheilk 23:297-321, 1910.
Siegrist A:  Konzentrierte Alkali- und Säurewirkung auf das Auge . Z Augenheilk 43:176-194, 1920.
Berezinskaya DI:  Augenverätzungen mit Säuren und Alkalien . Vestn Oftal 8:319-332, 1936.
Turss R, Friend J, Dohlman CH:  Effect of a corneal fluid barrier on the nutrition of the epithelium . Exp Eye Res 9:254-259, 1970.Article
Warburg O: Wasserstoffübertragende Fermente. Freiburg, Switzerland, Cantor GmbH, 1949.
Hohorst HJ, Kreutz FH, Bücher TH:  Ueber Metabolitgehalte und Metabolit-Konzentrationen in der Leber der Ratte . Biochem Z 332:18-46, 1959.
Reim M, Lax F, Lichte H, et al:  Steady state levels of glucose in the different layers of the cornea, aqueous humor, blood and tears in vivo . Ophthalmologica 154:39-50, 1967.Article
Reim M, Schmidt F:  Biochemische Veränderungen bei der Vereisung der Hornhaut in vivo: Ein Beitrag zur Kältetherapie . Klin Mbl Augenheilk 150:96-103, 1967.
Thoft RA, Friend J, Dohlman CH:  Corneal glucose flux . Arch Ophthal 85:467-472, 1971.Article
Dohlman CH, Brown SI, Martola E-L:  Replacement of the endothelium with alloplastic material: A new technique in corneal surgery . Trans Amer Acad Ophthal Otolaryng 71:851-864, 1967.