[Skip to Content]
[Skip to Content Landing]
May 1975

Circulating Immune ComplexesEffects on Ocular Vascular Permeability in the Rabbit

Author Affiliations

From the University of California School of Medicine, San Francisco.

Arch Ophthalmol. 1975;93(5):365-370. doi:10.1001/archopht.1975.01010020377013

Sudden transient, alteration in vascular permeability is producible in rabbit eyes by intravenous injection of large quantities of antigen, bovine γ-globulin (BGG), into immunized animals or by intravenous injection of large amounts of antigen-antibody complexes (BGG-antiBGG) prepared in antigen excess (20 to 25×) in normal rabbits. Change is measured by ocular accumulation of iodinated l 125 serum albumin, relative to that in heart blood, in intact eyes and separate anatomical compartments, in aqueous, in the anterior eye including (lens plus vitreous), and in the posterior segment. Primarily affected are vessels in the iridial portion of ciliary processes; edema is the primary finding. The same vessels are affected by intravenous injection of bacterial endotoxin, but compared with endotoxin, altered vascular permeability is short-lived and is not associated with formation of platelet plugs and intravenous fibrin strands.

Cochrane CG, Koffler D:  Immune complex disease in experimental animals and man . Adv Immunol 16:185-264, 1973.
Theodore FH, Lewson AC:  Bilateral iritis complicating serum sickness . Arch Ophthalmol 21:828-832, 1939.Article
Hoover RE:  Non-granulomatous uveitis: A complication of serum sickness . Am J Ophth 41:534-536, 1956.
Iga F:  Über heredreaktionen an den unberührten augen nach parenterakr zufuhr von artfremden serum . Klin Monatsbl Augenheilkd 84:449-478, 1930.
Wong VG, Anderson RR, McMaster PRB:  Endogenous immune uveitis: The role of serum sickness . Arch Ophthalmol 85:93-102, 1971.Article
Cochrane CG:  Studies on the localization of circulating antigen-antibody complexes and other macromolecules in vessels: I. Structural studies . J Exp Med 118:489-502, 1963.Article
Ayo C:  A toxic ocular reaction: I. New properties of Shwartzman toxins . J Immunol 46:113-126, 1943.
Levene RZ, Breinen GM:  Ocular effects of endotoxin . Arch Ophthalmol 61:568-577, 1959.Article
Howes EL Jr, Aronson SB, McKay DG:  Ocular vascular permeability: Effect of systemic administration of bacterial endotoxin . Arch Ophthalmol 84:360-367, 1970.Article
Firestone HJ, Werner M, Aronson SB:  Thin agarose gel immunologic methods . Am J Clin Pathol 51:769-776, 1969.
Lee L:  Antigen-antibody reaction in the pathogenesis of bilateral renal cortical necrosis . J Exp Med 117:365-375, 1963.Article
Brecker G, Cronkite EP:  Morphology and enumeration of human blood platelets . J Appl Physiol 3:365-377, 1950.
Kniker WT, Cochrane CG:  Pathogenetic factors in vascular lesions of experimental serum sickness . J Exp Med 122:83-98, 1965.Article
Meyer MM:  Complement and complement fixation , in Kabat EA, Meyer MM (eds): Experimental Immunochemistry . Springfield, Ill, Charles C Thomas, Publisher, 1961, pp 133-241.
Osier AG, Strauss JH, Mayer MM:  Diagnostic complement fixation, I. A. method . Am J Syphilis, Gonorrhea and VD 36:140-153, 1952.
Jensen PR, et al:  Mechanisms of host response in the eye: III. Intraocular protein transfer . Arch Ophthalmol 77:814-817, 1967Article
vol 1, pp 76-93.
Howes EL Jr, McKay DG:  Effect of cervical sympathectomy on the ocular response to systemic endotoxin . Proc Soc Exp Biol Med 139:839-844, 1972.Article
Becker EL, Austen KF:  Anaphylaxis , in Muller-Eberhard HJ, Miescher PA (eds): Immunopathology . New York, Grune & Stratton, Inc, 1967.
Movat HZ, Uriuhara T, Taichman WS, et al:  The role of PMN-leukocyte lysosomes in tissue injury, inflammation and hypersensitivity: VI. The participation of the PMN leukocyte and the blood platelet in systemic aggregate anaphylaxis . Immunology 14:637-648, 1968.
Weigle WO, Cochrane CG, Dixon FG:  Anaphylactogenic properties of soluble antigen-antibody complexes in the guinea pig and rabbit . J Immunol 85:469-477, 1960.
Duke-Elder S:  Diseases of the outer eye: Part 1, Conjunctiva , in Duke-Elder S (ed): System of Ophthalmology . St. Louis, CV Mosby & Co, 1965, vol 8, pp 33-39.
Howes EL Jr, McKay DG, Aronson SB:  An ultrastructural study of the ciliary process in the rabbit following systemic administration of bacterial endotoxin . Lab Invest 24:217-228, 1971.
Wegner K:  Regional differences in ultrastructures of the rabbit ciliary processes: The effect of anesthetic and fixation procedures . Invest Ophthalmol 6:177-191, 1967.
McCluskey RT, Benaceraff B, Potter JL, et al:  The pathologic effects of intravenously administered soluble antigen-antibody complexes: I. Passive serum sickness in mice . J Exp Med 111:181-194, 1960.Article
Cochrane CG, Hawkins D:  Studies on circulating immune complexes: III. Factors governing the ability of circulating complexes to localize in blood vessels . J Exp Med 127:137-154, 1968.Article
Benveniste J, Henson PM, Cochrane CG:  Leukocyte-dependent histamine release from rabbit platelets: The role of IgE, basophils, and a platelet-activating factor . J Exp Med 136:1356-1377, 1972.Article
Gamble CN, Aronson SB, Brescia FB:  Experimental uveitis: I. The production of recurrent immunologic (Auer) uveitis and its relationship to increased uveal vascular permeability . Arch Ophthalmol 84:321-330, 1970.Article
Pincus T, Haberkern R, Christian CL:  Experimental chronic glomerulonephritis . J Exp Med 127:819-831, 1968.Article
Cochrane CG, Muller-Eberhard HJ:  The derivation of two distinct anaphylatoxin activities from the third and fifth components of human complement . J Exp Med 127:371-386, 1968.Article
Waalkes TP, Weissbach H, Bozicevich J, et al:  Further studies on release of serotonin and histamine during anaphylaxis in the rabbit . Proc Soc Exp Biol Med 95:479-482, 1957.Article
Osier AG, Siraganian RP:  Immunologic mechanisms of platelet damage , in Kallos P, Waksman BH, de Weck A (eds): Progress in Allergy . Basel, Switzerland, S Karger AG, 1972, vol 16, pp 450-498.
Movat HZ:  The acute inflammatory reaction , in Movat HZ (ed): Inflammation, Immunity, and Hypersensitivity . New York, Harper & Row, 1971, pp 1-130.
Nies AS, Forsyth RP, Williams HE, et al:  Contribution of kinins to endotoxin shock in unanesthetized Rhesus monkeys . Circ Res 22:155-164, 1968.Article
Gewurz H, Snyderman R, Mergenhagen SE, et al:  Effects of endotoxin lipopolysaccharides on the complement system , in Kadis S, Weinbaum G, Ajl SJ (eds): Bacterial Endotoxins . New York, Academic Press, 1971, vol 5, pp 127-150.
Gaynor E:  Increased mitotic activity in rabbit endothelium after endotoxin . Lab Invest 24:318-320, 1971.