[Skip to Content]
[Skip to Content Landing]
Article
November 1976

McCarey-Kaufman Technique of Corneal Storage Before Penetrating Keratoplasties in RabbitsAn Evaluation

Author Affiliations

From the Pocklington Eye Transplantation Research Unit, Department of Clinical Ophthalmology, Institute of Ophthalmology, London. Dr Breslin is now with the Department of Ophthalmology, University of Toronto.

Arch Ophthalmol. 1976;94(11):1976-1979. doi:10.1001/archopht.1976.03910040682016
Abstract

• The endothelial function of rabbit corneas stored in modified tissue culture medium (McCarey-Kaufman medium) for periods of 4, 8, or 12 days was studied after penetrating keratoplasty using the specular microscope and the response to temperature reversal.

Our results indicate that rabbit donor corneas stored in McCarey-Kaufman (M-K) medium are suitable for penetrating keratoplasty after storage periods as long as 12 days.

The limitations of using graft clarity or the morphologic appearance of the endothelium as an index of the success of a penetrating keratoplasty in the rabbit are discussed.

References
1.
McCarey BE, Kaufman HE:  Improved corneal storage . Invest Ophthalmol 13:165-173, 1974.
2.
Bigar F, McCarey BE, Kaufman HE:  Improved corneal storage: Penetrating keratoplasties in rabbits . Exp Eye Res 20:219-222, 1975.Article
3.
Bigar F, Kaufman HE, McCarey BE, et al:  Improved corneal storage for penetrating keratoplasties in man . Am J Ophthalmol 79:115-120, 1975.
4.
Stark WJ, Maumanee AE, Kenyon KR:  Intermediate-term corneal storage for penetrating keratoplasty . Am J Ophthalmol 79:795-802, 1975.
5.
Breslin CW, Sherrard ES, Marshall J, et al:  An evaluation of the McCarey-Kaufman technique of corneal storage . Arch Ophthalmol 94:1545-1551, 1976.Article
6.
Dikstein S, Maurice DM:  The metabolic basis to the fluid pump in the cornea . J Physiol 22:29-41, 1972.
7.
Sherrard ES:  Anesthesia in rabbits . Vet Rec 78:253-254, 1966.
8.
Brightbill FS, Polack FM, Slappey T:  A comparison of two methods for cutting donor corneal buttons . Am J Ophthalmol 75:500-506, 1973.
9.
Sherrard ES:  Method of evaluating donor corneae for transplantation . Br J Ophthalmol 57:244-252, 1973.Article
10.
Sherrard ES:  An application of the temperature reversal effect: A possible method to evaluate donor corneae for penetrating keratoplasty . Exp Eye Res 15:667-675, 1973.Article
11.
Sherrard ES:  Full thickness keratoplasty in the rabbit: An unsatisfactory index of donor integrity . Exp Eye Res 18:135-142, 1974.Article
×