• Vitreous fluorophotometry was carried out in 99 juvenile-onset, insulindependent diabetics and 31 control subjects. The mean vitreous fluorescein concentration one hour after intravenous administration of fluorescein, 7 mg/kg, was 5.4 ± 0.3 ng/ml (mean ±SEM) for controls and 9.5 ± 0.4 ng/ml for diabetics (P <.005). Diabetic patients with or without background retinopathy, when randomly matched for age or duration of their disease, had similar vitreous fluorophotometry measurements. The breakdown of the blood-retinal barrier to fluorescein appears to be the earliest detectable ocular abnormality of diabetes.
Krupin T, Waltman SR, Oestrich C, Santiago J, Ratzan S, Kilo C, Becker B. Vitreous Fluorophotometry in Juvenile-Onset Diabetes Mellitus. Arch Ophthalmol. 1978;96(5):812-814. doi:10.1001/archopht.1978.03910050418002