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June 1978

Histochemistry of Human Extraocular Muscle

Author Affiliations

From the Department of Neurology (Dr Ringel, Messrs Barden and Kaiser), and the Department of Ophthalmology (Dr Wilson), University of Colorado Medical Center, Denver, and the Denver General Hospital (Dr Wilson). Mr Kaiser is now at the Washington University School of Medicine, St Louis.

Arch Ophthalmol. 1978;96(6):1067-1072. doi:10.1001/archopht.1978.03910050587020

• A reliable method for evaluating biopsy specimens of human extraocular muscles is presented to better understand the pathological response of these highly organized striated muscles. Three muscle fiber types and their distribution are described with morphological and histochemical measurements used commonly for limb muscle. The granular and fine fibers have single end plates and may be comparable to limb-twitch fibers (type 2 and type 1 fibers). The coarse fibers have multiple end plates and may correspond to multiple end plated tonic fibers found in avian and amphibian limb muscles.

The fibers of extraocular muscles are arranged in three concentric zones. Because of the zonal arrangement, a complete cross section should be evaluated in diseases of the ocular muscles to estimate any changes in fiber type distribution.

Rowland LP:  Progressive external ophthalmoplegia , in Vinken PJ, Bruyn GW (eds): Handbook of Clinical Neurology . Amsterdam, North-Holland Publishing Co, vol 22, chap 8, 1975.
Engel WK, Brooke MH:  Muscle biopsy as a clinical diagnostic aid , in Fields WS (ed): Neurological Diagnostic Techniques . London, WB Saunders Co Ltd, 1973, pp 1-57.
Drachman DA, Wetzel N, Wasserman M, et al:  Experimental denervation of ocular muscles . Arch Neurol 21:170-183, 1969.Article
Dietert SE:  The demonstration of different types of muscle fibers in human extraocular muscle by electronmicroscopy and cholinesterase staining . Invest Ophthalmol 4:51-63, 1965.
Brandt DE, Leeson CR:  Structural differences of fast and slow fibers in human extraocular muscles . Am J Ophthalmol 62:478-487, 1966.
Hess A:  The structure of slow and fast extrafusal muscle fibers in the extraocular muscles and their nerve endings in guinea pigs . J Cell Comp Physiol 58:63-80, 1961.Article
Mayr R:  Structure and distribution of fiber types in the external eye muscles of the rat . Tissue Cell 3:433-462, 1971.Article
Cheng K, Breinin CM:  A comparison of the fine structure of extraocular and interosseous muscles in the monkey . Invest Ophthalmol 5:535-549, 1966.
Kupfer C:  Motor innervation of extraocular muscle . J Physiol 153:522-526, 1960.
Salpeter MM, McHenry FA, Feng H:  Myoneural junctions in the extraocular muscles of the mouse . Anat Rec 179:201-224, 1974.Article
Alvarado JA, VanHorn C:  Muscle cell types of the cat inferior oblique , in Lennerstrand G (ed): Basic Mechanism of Ocular Motility and Their Clinical Implications . England, Pergamon Press, 1975, pp 15-43.
Harker DW:  The structure and innervation of sheep superior rectus and levator palpebris extraocular muscles . Invest Ophthalmol 11:956-969, 1972.
Durston JHJ:  Histochemistry of primate extraocular muscles and the changes of denervation . Br J Ophthalmol 58:193-216, 1974.Article
Miller JE:  Cellular organization of rhesus extraocular muscle . Invest Ophthalmol 6:18-39, 1967.
Yellin H:  Unique intrafusal and extraocular muscle fibers exhibiting dual actomyosin ATPase activity . Exp Neurol 25:153-163, 1969.Article
Ringel SP, Engel WK, Bender AN, et al: Histochemistry and acetylcholine receptor distribution in normal and denervated monkey extraocular muscles. Neurology, in press.
Jennekens FGI, Tan KEWP, Hoogenraad TV:  Enzyme-histochemistry of normal external ocular muscles . Ophthalmol (Basel) 173:326-327, 1976.Article
Kern R:  A comparative pharmacologic histologic study of slow and twitch fibers in the superior rectus muscle of the rabbit . Invest Ophthalmol 4:901-910, 1965.
Matyrushkin DP:  Phasic and tonic neuromotor units in the oculomotor apparatus of the rabbit . Sechenov Physiol J USSR 47:65, 1961.
Hess A, Pilar G:  Slow fibers in the extraocular muscles of the cat . J Physiol 169:780-798, 1963.
Bach-y-Rita P, Ito F:  In vivo studies on fast and slow muscle fibers in cat extraocular muscles . J Gen Physiol 49:1177-1198, 1966.Article
Yagi T:  Spontaneous and evoked behavior of single units in the oculomotor nucleus of the rabbit . Jpn J Physiol 24:305-316, 1974.Article
Hess A:  Vertebrate slow muscle fibers . Physiol Rev 50:40-62, 1970.
Ringel SP, Bender AN, Engel WK:  Alterations of acetylcholine receptors in human and experimental neuromuscular diseases . Arch Neurol 33:751-758, 1976.Article
Engel WK, Irwin RL:  A histochemicalphysiological correlation of frog skeletal muscle fibers . Am J Physiol 213:511-518, 1967.
Peachey LD, Huxley AF:  Structural identification of twitch and slow striated muscle fibers of the frog . J Cell Biol 13:177-180, 1962.Article
Fedde MR:  Electrical properties and acetylcholine sensitivity of singly and multiply innervated avian muscle fibers . J Gen Physiol 53:624-637, 1969.Article
Bennett MR, Pettigrew NG, Taylor RS:  The formation of synapses in reinnervated and cross reinnervated adult avian muscle . J Physiol 230:331-357, 1973.
Peachey LD, Takeichi M, Nag AC:  Muscle fiber types and innervation in adult cat extraocular muscle , in Milhorat AT (ed): Exploratory Concepts in Muscular Dystrophy II . New York, American Elsevier Publishing Co, 1974, pp 246-254.
Miller JE:  Aging changes in extraocular muscles , in Lennerstrand G (ed): Basic Mechanism of Ocular Motility and Their Clinical Implications . England, Pergamon Press, 1975, pp 47-61.