[Skip to Content]
[Skip to Content Landing]
Article
September 1979

Topical Antibiotic Therapy of Pseudomonas aeruginosa Keratitis

Author Affiliations

From the Departments of Ophthalmology (Drs Kupferman and Leibowitz) and Pharmacology (Dr Kupferman), Boston University School of Medicine.

Arch Ophthalmol. 1979;97(9):1699-1702. doi:10.1001/archopht.1979.01020020267016
Abstract

• The in vivo antibacterial effectiveness in the rabbit cornea of several commercially available ophthalmic antibiotic preparations was determined against a single strain of Pseudomonas aeruginosa isolated from a human corneal ulcer. Each antibiotic was instilled topically at hourly intervals, and the number of residual viable organisms in the cornea subsequently was ascertained. In vivo measurements correlated well with in vitro data and with generally held clinical impressions. Three antibiotics, gentamicin sulfate, polymyxin B sulfate, and colistin sulfate, suppressed corneal growth of P aeruginosa in commercially available concentrations. Gentamicin was slightly more effective than polymyxin B; both drugs were substantially more effective than colistin. Formulations of gentamicin and polymyxin B containing approximately four times the quantity of drug found in commercial preparations eliminated this P aeruginosa strain from the cornea much more rapidly than did the commercial preparations.

References
1.
Jones DB:  Early diagnosis and therapy of bacterial corneal ulcers . Int Ophthalmol Clin 13:1-29, 1973.Article
2.
Pettit TH:  Management of bacterial corneal ulcers , in Leopold IH, Burns RP (eds): Symposium on Ocular Therapy . New York, John Wiley & Sons Inc, 1976, vol 8, pp 57-65.
3.
Jones LF, Zakanycz JP, Thomas ET, et al:  Pyocin typing of Pseudomonas aeruginosa: A simplified method . Appl Microbiol 27:400-406, 1974.
4.
Bauer JD, Ackerman PG, Gelson T: Clinical Laboratory Methods . St Louis, CV Mosby Co, 1974, pp 658-664.
5.
Kupferman A, Leibowitz HM:  Quantitation of bacterial infection and antibiotic effect in the cornea . Arch Ophthalmol 94:1981-1984, 1976.Article
6.
Kupferman A, Leibowitz HM:  Topical antibiotic therapy of staphylococcal keratitis . Arch Ophthalmol 95:1634-1637, 1977.Article
7.
Wilson L:  Bacterial corneal ulcers , in Duane TD (ed): Clinical Ophthalmology . Hagerstown, Md, Harper & Row Publishers, 1976, vol 4, chap 18.
8.
Snedecor GW: Statistical Methods Applied to Experiments in Agriculture and Biology , ed 5. Ames, Iowa State University Press, 1956.
9.
Kucers A: The Use of Antibiotics . Philadelphia, JB Lippincott Co, 1972, p 141.
10.
Barker BM, Prescott F: Antimicrobial Agents in Medicine . Oxford, England, Blackwell Scientific Publications, 1973, p 244.
11.
Baum JL, Barza M, Shushan D, et al:  Concentration of gentamicin in experimental corneal ulcers: Topical vs subconjunctival therapy . Arch Ophthalmol 92:315-317, 1974.Article
12.
Weinstein LS, Perrin TS:  Treatment of scarlet fever with penicillin G administered orally three times a day . J Pediatr 37:844-853, 1950.Article
13.
Weinstein LS, Daikos G:  Treatment of scarlet fever with crystalline penicillin G administered orally or parenterally two times a day . Am Pract Dig Treatment 2:60-64, 1951.
14.
Davis SD, Sarff LD, Hyndiuk RA:  Antibiotic therapy of experimental Pseudomonas keratitis in guinea pigs . Arch Ophthalmol 95:1638-1643, 1977.Article
×