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Article
March 1980

Treatment of Experimental Herpetic Interstitial Keratitis With Medroxyprogesterone

Author Affiliations

From the Department of Cornea Research, Eye Research Institute of Retina Foundation (Drs Lass, Berman, Campbell, Pavan-Langston, and Ms Gage) and the Department of Ophthalmology, Harvard Medical School (Drs Lass, Berman, Campbell, and Pavan-Langston), Boston. Dr Lass is now with University Hospitals, Cleveland.

Arch Ophthalmol. 1980;98(3):520-527. doi:10.1001/archopht.1980.01020030516018
Abstract

• A model of herpes simplex interstitial keratitis was developed in rabbits sensitized with live herpes simplex virus (HSV) type 2 and challenged intrastromally with live virus. This model was used to evaluate the effects of subconjunctival medroxyprogesterone acetate on the course of the disease and on collagenase levels in the treated corneas. Whether treated prophylactically or therapeutically, the medroxyprogesterone-treated groups had substantially less stromal infiltration and neovascularization than the controls. The clinical effects corresponded with a marked reduction in the polymorphonuclear leukocyte infiltrate histologically, and the suppression of latent and active collagenase activity in the treated corneas cultured in vitro. However, epithelial disease was exacerbated in prophylactically treated animals. Medroxyprogesterone appears to be useful in the treatment of herpetic interstitial keratitis as an anti-inflammatory agent as well as an inhibitor of collagenase production but, like the corticosteroids, it can exacerbate epithelial disease.

References
1.
Pavan-Langston D:  Diagnosis and management of herpes simplex ocular infection . Int Ophthalmol Clin 15:19-35, 1975.Article
2.
Dawson C, Togni B, Moore T:  Structural changes in chronic herpetic keratitis: Studied by light and electron microscopy . Arch Ophthalmol 79:740-747, 1968.Article
3.
Henson D, Helmsen R, Becker KE, et al:  Ultrastructural localization of herpes simplex virus antigens on rabbit corneal cells using sheep antihuman IgG antihorse ferritin hybrid antibodies . Invest Ophthalmol 13:819-827, 1974.
4.
Metcalf J, Helmsen R:  Immunoelectron microscopic localization of herpes simplex virus antigens in rabbit cornea with antihuman IgG antiferritin hybrid antibodies . Invest Ophthalmol 16:779-786, 1977.
5.
Meyers R, Pettit T:  Corneal immune response to herpes simplex virus antigens . J Immunol 110:1575-1590, 1975.
6.
Binder P:  Herpes simplex keratitis . Surv Ophthalmol 21:313-330, 1970.Article
7.
Dawson C, Togni B:  Herpes simplex eye infections: Clinical manifestations, pathogenesis, and management . Surv Ophthalmol 21:121-135, 1976.Article
8.
Kaufman H:  Practical considerations in the selection of anti-inflammatory agents . Trans Am Acad Ophthalmol Otolaryngol 79:89-94, 1975.
9.
Koob TJ, Jeffrey JJ:  Hormonal regulation of collagen degradation in the uterus: Inhibition of collagenase expression by progesterone and cyclic AMP . Biochim Biophys Acta 345:61, 1974.Article
10.
Halme J, Woessner JF Jr:  Effect of progesterone on collagen breakdown and tissue collagenolytic activity in the involuting rat uterus . J Endocrinol 66:357-362, 1975.Article
11.
Wahl LM, Wahl SM, Mergenhagen SE, et al:  Collagenase production by lymphokine-activated macrophages . Science 189:261-263, 1975.Article
12.
Newsome D, Gross J:  Prevention by medroxyprogesterone of perforation in the alkali-burned rabbit cornea: Inhibition of collagenolytic activity . Invest Ophthalmol 16:21-31, 1977.
13.
Cavanagh HD:  Herpetic ocular disease: Therapy of persistent epithelial defects . Int Ophthalmol Clin 15:67-88, 1975.Article
14.
McCulley JP, Slansky HH, Pavan-Langston D, et al:  Collagenolytic activity in experimental herpes simplex keratitis . Arch Ophthalmol 84:516-519, 1970.Article
15.
Campbell R, Pavan-Langston D, Lass J, et al: Collagenase levels in a new model of experimental herpetic interstitial keratitis. Arch Ophthalmol, to be published.
16.
Berman M, Manabe R, Davison P:  Tissue collagenase: A simplified semiquantitative enzyme assay . Anal Biochem 54:522-534, 1973.Article
17.
Rojkind M, Gonzalez E:  An improved method for determining specific radioactivities of proline-14C and hydroxyproline-14C in collagen and in noncollagenous protein . Anal Biochem 57:1-7, 1974.Article
18.
Berman M, Leary R, Gage J:  Latent collagenase in the ulcerating rabbit cornea . Exp Eye Res 25:435-446, 1977.Article
19.
Pavan-Langston D, Langston RHS, Geary PA:  Prophylaxis and therapy of experimental ocular herpes simplex: Comparison of idoxuridine, adenine arabinoside, and hypoxanthine arabinoside . Arch Ophthalmol 92:417-421, 1974.Article
20.
Robertson PB, Ryel RB, Taylor RE, et al:  Collagenase: Localization in polymorphonuclear leukocyte granules in the rabbit . Science 177:64-65, 1972.Article
21.
Burleigh MC:  Degradation of collagen by non-specific proteinases , in Barrett AJ (ed): Proteinases in Mammalian Cells and Tissues . New York, Elsevier North Holland Inc, 1977, pp 285-309.
22.
Kenyon K, Berman M, Rose J, et al:  Prevention of stromal ulceration in the alkali-burned rabbit cornea by glued-on contact lens: Evidence for the role of polymorphonuclear leukocytes in collagen degradation . Invest Ophthalmol Vis Sci 18:570-587, 1979.
23.
Hook R, Hook C, Brown S:  Fibroblast collagenase partial purfication and characterization . Invest Ophthalmol 12:771-776, 1973.
24.
Slansky H, Dohlman C:  Collagenase and the cornea . Surv Ophthalmol 14:402-416, 1970.
25.
Berman M, Cavanagh HD, Gage J:  Regulation of collagenase activity in the ulcerating cornea by cyclic AMP . Exp Eye Res 22:209-218, 1976.Article
26.
Koob T, Jeffrey J, Eisen A:  Regulation of human skin collagenase activity by hydrocortisone and dexamethasone in organ culture . Biochem Biophys Res Commun 61:1083-1088, 1974.Article
27.
Lemp MA:  Cornea and sclera . Arch Ophthalmol 94:473-490, 1976.Article
28.
Takahashi GH, Leibowitz HM, Kibrick S:  Topically applied steroids in active herpes simplex keratitis . Arch Ophthalmol 85:350-354, 1971.Article
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