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Article
January 1981

Feulgen-Positive Deposits in Retinoblastoma Incidence, Composition, and Ultrastructure

Author Affiliations

From the Department of Ophthalmology, University of Washington, Seattle (Dr Bunt), and the Georgiana Dvorak Theobald Laboratory of Eye Pathology, Department of Ophthalmology, University of Illinois, Chicago (Dr Tso).

Arch Ophthalmol. 1981;99(1):144-150. doi:10.1001/archopht.1981.03930010146021
Abstract

• The incidence of hematoxyphilic extracellular deposits in 159 cases of retinoblastoma was found to be 18%. Six other features of these tumors also were examined. In the majority of eyes with these deposits, the tumors were composed solely of anaplastic cells. None was well differentiated with preponderantly rosette formation. The hematoxyphilic deposits were Feulgen-positive, and this staining was diminished but not abolished by pretreatment with purified deoxyribonuclease (DNase), which did abolish nuclear staining. Feulgen staining of the deposits was totally blocked by pronase—DNase pretreatment—suggesting that the deposits may represent DNA-protein complexes. Ultrastructurally, these deposits were electron dense and finely vacuolar. The deposits may originate from degenerating tumor cell nuclei, and the possibility that they may represent immune complexes warrants future study in view of occasional spontaneous regression of this tumor.

References
1.
Rosenmann E, Boye ES, Lachance RC:  DNA coating of blood vessels: A nonspecific finding in cellular malignant tumors with extensive necrosis . Isr J Med Sci 13:1129-1130, 1977.
2.
Reese AB:  Retinoblastoma and other neuroectodermal tumors of the retina , in Tumors of the Eye , ed 3. Hagerstown, Md, Harper & Row Publishers Inc, 1976, pp 90-132.
3.
McKeown F:  Oat-cell carcinoma of the oesophagus . J Pathol Bacteriol 64:889-891, 1952.Article
4.
Ogilvie RF: Pathological Histology , ed 5. Baltimore, Williams & Wilkins Co, 1957, p 13.
5.
Azzopardi JG:  Oat-cell carcinoma of the bronchus . J Pathol Bacteriol 78:513-519, 1959.Article
6.
Mullaney J:  DNA in retinoblastoma . Lancet 2:918-919, 1968.Article
7.
Mullaney J:  Retinoblastomas with DNA precipitation . Arch Ophthalmol 82:454-456, 1969.Article
8.
Mullaney J:  Retinoblastoma: A review and some new aspects . Irish J Med Sci 2:57-63, 1969.Article
9.
Mullaney J:  Reply on DNA precipitation . Arch Ophthalmol 84:547, 1970.Article
10.
Bernardino V Jr, Salceda SR:  DNA precipitation . Arch Ophthalmol 84:547, 1970.Article
11.
Datta BN:  DNA coating of blood vessels in retinoblastomas . Am J Clin Pathol 62:94-96, 1974.
12.
Ahmed A:  Some ultrastructural observations of haematoxyphil vascular changes in oatcell carcinoma . J Pathol 112:1-3, 1974.Article
13.
Stowe GC III, Zakov ZN, Albert DM, et al:  Vascular basophilia in ocular and orbital tumors . Invest Ophthalmol Vis Sci 18:1068-1075, 1979.
14.
Pearse AGE:  Nucleic acids and nucleoproteins , in Histochemistry, Theoretical and Applied , ed 3. Edinburgh, Churchill Livingston, 1968, vol 1, pp 148-293.
15.
Pearse AGE:  Enzymes as histochemical reagents , in Histochemistry, Theoretical and Applied , ed 3. Baltimore, Williams & Wilkins Co, 1972, vol 2, pp 1023-1025.
16.
Kawalec M, Gajewski A:  Deoxyribonuclease I purification from protease contaminations . Folia Histochem Cytochem 15:181-185, 1977.
17.
Nakao F, Yoo JH, Uga S:  Unusual intercellular dense materials in the non-tumorous parts of the retina with retinoblastoma . Jpn J Ophthalmol 20:159-165, 1976.
18.
Sang DN, Albert DM:  Recent advances in the study of retinoblastoma , in Peyman GA, Apple DJ, Sanders DR (eds): Intraocular Tumors . New York, Appleton-Century-Crofts, 1977, pp 285-329.
19.
Char DH, Christensen M, Goldberg L, et al:  Immune complexes in retinoblastoma . Am J Ophthalmol 86:395-399, 1978.
20.
Khodadoust AA, Roozitalab HM, Smith RE, et al:  Spontaneous regression of retinoblastoma . Surv Ophthalmol 21:467-478, 1977.Article
21.
Mullaney J: Histochemical immunological study of retinoblastoma. Jpn J Ophthalmol, to be published.
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