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Article
March 1982

Fluorescein Binding to Normal Human Serum Proteins Demonstrated by Equilibrium Dialysis

Author Affiliations

From the Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Medical School, Philadelphia.

Arch Ophthalmol. 1982;100(3):484-487. doi:10.1001/archopht.1982.01030030486024
Abstract

• The binding of fluorescein to normal human serum proteins in a physiologic solvent at 37 °C was measured by equilibrium dialysis. Human serum contained 3.28 x 10-3M concentration fluoresceinbinding sites, with an average association constant at 37 °C of 0.54 X 104M-1. The percentage of total fluorescein bound by human serum proteins ranged from 83% to 53% when the total fluorescein concentration ranged from 6.9 X 10-5 to 6.2 X 10-3M.

References
1.
Novotmy HR, Alvis DL:  A method of photographing fluorescence in circulating blood in the human retina . Circulation 1961;24:82-86.Article
2.
Wessing A:  Biochemical properties of fluorescein , in Fluorescein Angiography of the Retina . St Louis, CV Mosby Co, 1969, pp 13-14.
3.
Lange K, Boyd LJ:  Use of fluorescein method in establishment of diagnosis and prognosis of peripheral vascular diseases . Arch Intern Med 1944;74:175-184.Article
4.
Hodge JY, Dollery CT:  Retinal soft exudates: A clinical study by colour and fluorescence photography . Q J Med 1964;33:117-130.
5.
Laurence DJR:  A study of the absorption of dyes on bovine serum albumin by the method of polarization of fluorescence . Biochem J 1952; 51:168-180.
6.
Andersson LO, Rehnström A, Eaker DL:  Studies on 'non-specific' binding: The nature of the binding of fluorescein to bovine serum albumin . Eur J Biochem 1971;20:371-380.Article
7.
Ianacone DC, Felberg NT, Federman JL:  Tritiated fluorescein binding to normal human plasma proteins . Arch Ophthalmol 1980;98:1643-1645.Article
8.
Karush F, Sonenberg M:  Interaction of homologous alkyl sulfates with bovine serum albumin . J Am Chem Soc 1949;71:1369-1376.Article
9.
Haschemeyer RH, Haschemeyer AE:  Method based on charge or charge distribution , in Haschemeyer RH, Haschemeyer AE (eds): Proteins: A Guide to Study by Physical and Chemical Methods . New York, John Wiley & Sons Inc, 1973, pp 254-268.
10.
Karush F:  The interaction of purified antibody with optically isomeric haptens . J Am Chem Soc 1956;78:5519-5526.Article
11.
Klotz I:  The application of the law of mass action to binding by proteins: Interactions with calcium . Arch Biochem 1946;9:109-117.
12.
Scatchard G, Scheinberg IH, Armstrong SH:  Physical chemistry of protein solutions: IV. The combination of human serum albumin with chloride ion . J Am Chem Soc 1950;72:535-540.Article
13.
Rockey JH:  Equine antihapten antibody: The subunits and fragments of anti-β-lactoside antibody . J Exp Med 1967;125:249-275.Article
14.
Wu WH, Rockey JH:  Antivasopressin antibody: Characterization of high-affinity rabbit antibody with limited association constant heterogeneity . Biochemistry 1969;8:2719-2728.Article
15.
Guyton AC (ed): Textbook of Medical Physiology . Philadelphia, WB Saunders Co, 1976, pp 393-394.
16.
Dollery CT, Hodge JV, Engel M:  Studies of the retinal circulation with fluorescein . Br Med J 1962;2:1210-1215.Article
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