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June 1982

Source of Subretinal Fluid on the Basis of Ascorbate Analyses

Author Affiliations

From the Departments of Ophthalmology (Drs van Heuven, Lam, and Ray) and Biochemistry (Dr Lam), Albany (NY) Medical College. Dr van Heuven is now with the University of Texas, Austin.

Arch Ophthalmol. 1982;100(6):976-978. doi:10.1001/archopht.1982.01030030984017

• Biochemical analyses of subretinal fluid revealed a consistently high ascorbate level in the subretinal fluid of patients with rhegmatogenous retinal detachment. The average values and SDs of ascorbate in anterior chamber aqueous humor, subretinal fluid, and blood were 14.7 ± 1.8, 27.4 ± 2.1, and 1.8 ± 0.2 mg/dL, respectively. The ascorbate concentration in subretinal fluid was always higher than that in aqueous humor. The high ascorbate level in subretinal fluid led to the hypothesis that aqueous humor contributes to the formation of subretinal fluid. Presumably the constant absorption of subretinal fluid by the choroid directs a portion of the aqueous humor from the posterior chamber into the subretinal space. The posterior movement of aqueous humor causes reduced ascorbate concentration in the anterior chamber and relative hypotony of eyes with rhegmatogenous retinal detachment. Closing the retinal break results in an interruption of the posterior movement of aqueous humor and rapid absorption of the remaining subretinal fluid by the choroid.

Beigelman MN:  Acute hypotony in retinal detachment . Arch Ophthalmol 1929;1:463-467.Article
Syrdalen P:  Intraocular pressure and ocular rigidity in patients with retinal detachment: I. Preoperative study . Acta Ophthalmol 1970;48:1024-1035.Article
Dobbie JG:  A study of the intraocular fluid dynamics in retinal detachment . Arch Ophthalmol 1963;69:159-164.Article
Rousseau AP, Regan CDJ:  Pressure cup studies in eyes with retinal detachment . Arch Ophthalmol 1965;73:803-809.Article
Foulds WS:  Experimental detachment of the retina and its effect on the intraocular fluid dynamics . Mod Probl Ophthalmol 1969;8:51-63.
Van Heuven WAJ, Lam KW: Aqueous humor as the origin of subretinal fluid. Read before a meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 4, 1978.
Lam KW, Lee PF:  Analyses of ascorbate concentration in the aqueous humor by high pressure liquid chromatography . Invest Ophthalmol Vis Sci 1975;14:947-950.
Lowry OH, Rosebrough NJ, Farr AL, et al:  Protein measurement with the folin phenol reagent . J Biol Chem 1951;193:265-275.
Gloor BP, discussant in Dufour R:  Technique of subretinal fluid drainage . Mod Probl Ophthalmol 1975;15:219.
Leaver PK, Chester GH, Saunders SH:  Factors influencing absorption of subretinal fluid . Br J Ophthalmol 1976;60:557-560.Article
Kinsey VE:  Transfer of ascorbic acid and related compounds across the blood-aqueous barrier . Am J Ophthalmol 1947;3:1262-1266.
Moses RA:  Intraocular pressure , in Moses RA (ed): Adler's Physiology of the Eye . St Louis, CV Mosby Co, 1975, chap 6.