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Article
November 1982

Intraocular Penetration of AmikacinIris Binding and Bioavailability

Author Affiliations

From the Departments of Ophthalmology (Dr Eiferman) and Medicine (Dr Stagner), University of Louisville School of Medicine and the Veterans Administration Medical Center.

Arch Ophthalmol. 1982;100(11):1817-1819. doi:10.1001/archopht.1982.01030040797018
Abstract

• The penetration of amikacin sulfate into the anterior chamber of the human eye was determined by radioimmunoassay. Bactericidal concentrations of amikacin were not achieved by topical or intravenous administration. Subconjunctival injection did not produce consistent bactericidal concentrations of amikacin in aqueous humor. Poor corneal penetration and subsequent tight binding to iris pigment are responsible for these observations. Tissue or pigment binding is adsorptive, nonspecific, and readily reversible. Amikacin released after being bound retains its bactericidal potency.

References
1.
Dupont HL, Spink WW:  Infection due to Gram-negative organisms . Medicine 1969;48:307-332.Article
2.
Valdivieso M, Feld R, Rodriguez V, et al:  Amikacin therapy of infections in neutropenic patients . Am J Med Sci 1975;270:453-463.Article
3.
Potts AM:  The reaction of uveal pigment in vitro with polycyclic compounds . Invest Ophthalmol 1964;3:405-416.
4.
Rodbard D:  Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays . Clin Chem 1974;20:1255-1270.
5.
Nelson P, Peyman GA, Bennett TO:  BB-K8: A new aminoglycoside of intravitreal injection in bacterial endophthalmitis . Am J Ophthalmol 1974;78:82-89.
6.
Oishi M, Nakatsui T, Nishizuka K, et al:  Ophthalmic use of amikacin . Jpn J Antibiot 1974;27:238-243.
7.
Young P, Barza M, Kane A, et al:  Radioactive and bioassay of intraocular antibiotics . Arch Ophthalmol 1977;97:717-720.Article
8.
Barza M, Baum J, Kane A:  Inhibition of antibiotic activity in vitro by synthetic melanin . Antimicrob Agents Chemother 1976;10:509-512.
9.
Mikkelson TJ, Chrai SS, Robinson JR:  Altered bioavailability of drugs in the eye due to drug-protein interaction . J Pharm Sci 1973;62:1648-1653.Article
10.
Mikkelson TJ, Chrai SS, Robinson JR:  Competitive inhibition of drug-protein interaction in eye fluid and tissue . J Pharm Sci 1973;62:1942-1945.Article
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