[Skip to Content]
[Skip to Content Landing]
March 1983

Radial KeratotomyEffect on Cornea and Aqueous Humor Physiology in the Rabbit

Author Affiliations

From the Departments of Ophthalmology (Drs Hull, Farkas, and Green, Mss Laughter and Bowman, and Mr Elijah) and Physiology (Dr Green), Medical College of Georgia, Augusta.

Arch Ophthalmol. 1983;101(3):479-481. doi:10.1001/archopht.1983.01040010479032

• Radial keratotomy may cause anatomical damage to the rabbit corneal endothelium. To determine if physiological and functional alterations occur, radial keratotomy was performed on rabbit corneas using eight incisions with sparing of a 3.5-mm central pupillary area. Cornea endothelial membrane permeabilities were determined at various times up to ten weeks following the procedure using simultaneous flux determinations of tritiated inulin and dextran labeled with radioactive carbon on isolated corneas. At all times after radial keratotomy, there was no probable physiologically important change in corneal endothelial permeability to either of the labeled compounds. Fluorophotometry performed in a second group of animals at varying intervals following radial keratotomy showed no probable physiologically important change in endothelial fluorescein permeability. Aqueous humor turnover rate was reduced 16% and 29% at one week and nine to ten weeks, respectively, following radial keratotomy.

Fyodorov SN, Durnev VV:  Operation of dosaged dissection of corneal circular ligament in cases of myopia of mild degree . Ann Ophthalmol 1979;11:1885-1890.
Fyodorov SN, Durnev VV:  Surgical correction of complicated myopic astigmatism by means of dissection of circular ligament of cornea . Ann Ophthalmol 1981;13:115-118.
Bores LD, Myers W, Cowden J:  Radial keratotomy: An analysis of the American experience . Ann Ophthalmol 1981;13:941-948.
Steel D, Jester JV, Salz J, et al:  Modification of corneal curvature following radial keratotomy in primates . Ophthalmology 1981;88:747-754.Article
Jester JV, Steel D, Salz J, et al:  Radial keratotomy in non-human primate eyes . Am J Ophthalmol 1981;92:153-171.
Yamaguchi T, Kaufman HE, Fukushima A, et al:  Histologic and electron microscopic assessment of endothelial damage produced by anterior radial keratotomy in the monkey cornea . Am J Ophthalmol 1981;92:313-327.
Yamaguchi T, Polack FM, Valenti J, et al:  Endothelial damage after anterior radial keratotomy . Arch Ophthalmol 1981;99:2151-2158.Article
Kim JH, Green K, Martinez M, et al:  Solute permeability of the corneal endothelium and Descemet's membrane . Exp Eye Res 1971;12:231-238.Article
Jones RF, Maurice DM:  New methods of measuring the rate of aqueous flow in man with fluorescein . Exp Eye Res 1966;5:208-220.Article
Waltman SR, Kaufman HE:  A new objective slit lamp fluorophotometer . Invest Ophthalmol Vis Sci 1970;9:247-249.
Green K, Symonds CM, Elijah RD, et al:  Water soluble marijuana-derived material: Pharmacological actions in rabbit and primate . Curr Eye Res 1982;1:599-608.Article
Yablonski ME, Zimmerman TJ, Waltman SR, et al:  A fluorophotometric study of the effect of topical timolol on aqueous dynamics . Exp Eye Res 1978;27:135-142.Article
Burns RR, Bourne WM, Brubaker RF:  Endothelial function in patients with cornea guttata . Invest Ophthalmol Vis Sci 1981;20:77-85.
Coakes RL, Brubaker RF:  Methods of measuring aqueous humor flow and corneal endothelial permeability using a fluorophotometery nomegram . Invest Ophthalmol Vis Sci 1979; 18:288-302.
Brubaker RF, Coakes RL, Bourne WM:  Effect of timolol on the permeability of corneal endothelium . Ophthalmology 1979;86:108-111.Article
Hull DS, Green K, Boyd M, et al:  Corneal endothelium bicarbonate transport and the effect of carbonic anhydrase inhibitors on endothelial permeability and fluxes and corneal thickness . Invest Ophthalmol Vis Sci 1977;16:883-892.
Hull DS, Green K, Bowman K:  Corneal endothelial permeability following storage in moist chamber of MK medium . Acta Ophthalmol 1979;57:999-1003.Article
McCarey BE, Edelhauser HF, van Horn DL:  Functional and structural changes in the corneal endothelium during in vitro perfusion . Invest Ophthalmol Vis Sci 1973;12:410-417.
Maffly RH, Hays RM, Lamdin E, et al:  The effect of neurohypophyseal hormones on the permeability of the toad bladder to urea . J Clin Invest 1960;39:630-641.Article
Van Horn DL, Sendele DD, Seideman S, et al:  Regenerative capacity of the corneal endothelium in rabbit and cat . Invest Ophthalmol Vis Sci 1977;16:597-613.
Khodadoust AA, Green K:  Physiological function of regenerating endothelium . Invest Ophthalmol Vis Sci 1976;15:96-101.