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Article
April 1984

Selection of Therapeutic Agents for Intraocular Proliferative DiseaseCell Culture Evaluation

Author Affiliations

From the Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine.

Arch Ophthalmol. 1984;102(4):598-604. doi:10.1001/archopht.1984.01040030470029
Abstract

• A variety of antimetabolites, steroids, and nonsteroidal anti-inflammatory agents were tested for their ability to inhibit rabbit dermal and conjunctival fibroblast proliferation in cell culture. Doxorubicin hydrochloride and fluorouracil produced notable inhibition in concentrations of less than 1 mg/L. Meclofenamate sodium and indomethacin produced notable inhibition at concentrations of 11 and 40 mg/L, respectively. Dexamethasone sodium phosphate and triamcinolone acetonide produced inhibition at 200 and 150 mg/L, respectively, but paradoxically increased proliferation almost twofold at concentrations ranging from 1 to 30 mg/L under identical culture conditions. Methotrexate sodium demonstrated only limited effectiveness. This assay system may be a useful approach to drug selection in the treatment of a variety of ocular proliferative disorders. Fluorouracil may prove to be of significant value in the treatment of intraocular proliferative disorders.

References
1.
Machemer R:  Massive periretinal proliferation: A logical approach to therapy . Trans Am Ophthalmol Soc 1977;75:556-586.
2.
Cleary PE, Minckler DS, Ryan SJ:  Ultrastructure of traction retinal detachment in rhesus monkey eyes after a posterior penetrating ocular injury . Am J Ophthalmol 1980;90:829-845.
3.
Ruhmann AG, Berliner DI:  Influence of steroids on fibroblasts: II. The fibroblast as an assay system for topical anti-inflammatory potency of corticosteroids . J Invest Dermatol 1967;49:123-130.Article
4.
Ogawa M, Bergsagel DE, McCulloch EA:  Chemotherapy of mouse myeloma: Quantitative cell cultures predictive of response in vivo . Blood 1973;41:7-15.
5.
Hial V, DeMello MCF, Horakova Z, et al:  Antiproliferative activity of anti-inflammatory drugs in two mammalian cell culture lines . J Pharmacol Exp Ther 1977;202:446-454.
6.
Ophir A, Blumenkranz M, Claflin A:  Experimental intraocular proliferation and neovascularization . Am J Ophthalmol 1982;94:450-457.
7.
Ponec M, DeHaas C, Bachra BN, et al:  Effects of glucocorticosteroids on cultured human skin fibroblasts: III. Transient inhibition of cell proliferation in the early growth stages and reduced susceptibility in later growth stages . Arch Dermatol Res 1979;265:219-227.Article
8.
Priestley GC:  Effects of corticosteroids on the growth and metabolism of fibroblasts cultured from human skin . Br J Dermatol 1978; 99:253-261.Article
9.
Runikas JO, McLean DI, Stewart WD:  Growth rate of cultured human fibroblasts increased by glucocorticoids . J Invest Dermatol 1978;70:348-351.Article
10.
Blumenkranz M, Ophir A, Claflin A:  A pharmacologic approach to non-neoplastic intraocular proliferation , abstracted. Invest Ophthalmol Vis Sci 1981;20:200.
11.
Clarkson JM, Humphrey RM:  The effect of Adriamycin on cell cycle progression and DNA replication in Chinese hamster ovary cells . Cancer Res 1977;37:200-205.
12.
Elion GB, Hitchings GH:  Metabolic basis for the actions of analogs of purines and pyrimidines . Adv Chemotherapy 1965;2:91-177.
13.
Neupert G, Muller P:  Growth inhibition and morphological changes caused by indomethacin in fibroblasts in vitro . Exp Pathol 1975; 11:1-9.
14.
Balk SD, Whitfield JF, Youdale T, et al:  Roles of calcium, serum, plasma, and folic acid in the control of proliferation of normal and Rous sarcoma virus-infected chicken fibroblasts . Proc Natl Acad Sci USA 1973;70:675-679.Article
15.
Algvere P, Koch E:  Experimental fibroplasia in the rabbit vitreous: Retinal detachment induced by autologous fibroblasts . Graefes Arch Clin Exp Ophthalmol 1976;199:215-222.Article
16.
Sugita G, Tano Y, Machemer R, et al:  Intravitreal autotransplantation of fibroblasts . Am J Ophthalmol 1980;89:121-130.
17.
Radtke ND, Tano Y, Chandler D, et al:  Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits . Am J Ophthalmol 1981;91:76-87.
18.
Finkelstein D, Brem S, Patz A, et al:  Experimental retinal neovascularization induced by intravitreal tumors . Am J Ophthalmol 1977; 83:660-664.
19.
Burke JM, Kower HS:  Collagen synthesis by rabbit neural retina in vitro and in vivo . Exp Eye Res 1980;31:213-226.Article
20.
Fensleau A, Wallis K, Kaiser D:  Studies on a new class of potential antiangiogenic agents: Nucleoside antimetabolite drugs . Microvasc Res 1981;22:58-63.Article
21.
Bunt AH, Lund RD:  Vinblastine-induced blockage of orthograde and retrograde axonal transport of protein in retinal ganglion cells . Exp Neurol 1974;45:288-297.Article
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