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Article
April 1984

Ocular Immunopathologic Findings of Experimental Onchocerciasis

Author Affiliations

From the Department of Clinical Veterinary Medicine, University of Cambridge, England (Drs Donnelly and Soulsby); the Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania School of Medicine, Philadelphia (Drs Donnelly and Rockey); and the London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans, England (Dr Bianco).

Arch Ophthalmol. 1984;102(4):628-634. doi:10.1001/archopht.1984.01040030500036
Abstract

• Ocular immunopathologic responses of inbred guinea pigs infected with Onchocerca microfilariae from domesticated animals were studied as a laboratory model of human ocular onchocerciasis. A single intracorneal infection of normal guinea pigs with microfilariae produced only minimal ocular lesions. In contrast, intracorneal infection of guinea pigs previously immunized by systemic infection with microfilariae produced intense corneal and uveal inflammation. Transfer of splenic lymphocytes from immunized donors to syngeneic normal recipients substituted effectively for the active immunization. Cell recipients produced marked corneal inflammatory reactions when challenged by a single intracorneal infection. Fresh and cryopreserved microfilariae produced identical reactions. The corneal inflammatory infiltrates were composed primarily of eosinophils, neutrophils, and plasma cells and resembled human onchocercal keratitis. Diethylcarbamazine citrate administration after a challenge intracorneal infection increased the severity of the corneal inflammatory response in immunized animals.

References
1.
Connor DH:  Onchocerciasis . N Engl J Med 1978;298:379-380.Article
2.
Nelson GS:  Onchocerciasis . Adv Parasitol 1970;8:173-224.
3.
Thylefors B:  Ocular onchocerciasis . Bull WHO 1978;56:63-73.
4.
Garner A, Duke BOL, Anderson J:  A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-Savanna strains of Onchocerca volvulus from Cameroon: II. The pathology . Tropenmed Parasitol 1973;24:385-396.
5.
Duke BOL, Garner A:  Reactions to subconjunctival inoculation of Onchocerca volvulus microfilariae in pre-immunized rabbits . Tropenmed Parasitol 1975;26:435-448.
6.
Garner A:  Laboratory research on the pathogenesis of ocular onchocerciasis . Trans Ophthal Soc UK 1980;100:179-185.
7.
Cello RM:  Ocular onchocerciasis in the horse . Equine Vet J 1971;3:148-154.Article
8.
Schmidt GM, Krehbiel JD, Coley SC, et al:  Equine ocular onchocerciasis: Histopathologic study . Am J Vet Res 1982;43:1371-1375.
9.
Donnelly JJ, Rockey JH, Bianco AE, et al:  Aqueous humor and serum IgE antibody in experimental ocular Onchocerca infection of guinea pigs . Ophthalmic Res 1983;15:61-67.Article
10.
Bianco AE, Ham P, El Sinnary K, et al:  Large-scale recovery of Onchocerca microfilariae from naturally infected cattle and horses . Trans R Soc Trop Med Hyg 1980;74:109-110.Article
11.
Ottley ML, Moorhouse DE:  Equine onchocerciasis . Aust Vet J 1978;54:545.Article
12.
Ham P, Townson S, James ER, et al:  An improved technique for cryopreservation of Onchocerca microfilariae . Parasitology 1981; 83:139-146.Article
13.
Millonig G:  Advantages of a phosphate buffer for osmium tetroxide solutions in fixation . J Appl Physiol 1961;32:1637.
14.
Muller R:  The identification of Onchocerca , in Taylor AEB, Muller R (eds): Problems in the Identification of Parasites and Their Vectors: Seventeenth Symposium of the British Society for Parasitology . Oxford, England, Blackwell Scientific Publications, 1979, pp 175-206.
15.
Taichman NS:  Ultrastructure of guinea pig mast cells . J Ultrastruct Res 1970;32:284-292.Article
16.
Taichman NS:  Ultrastructural alterations in guinea pig mast cells during anaphylaxis . Int Arch Allergy 1971;40:934-942.Article
17.
Rockey JH, Donnelly JJ, Stromberg BE, et al:  Immunopathology of ascarid infection of the eye: Role of IgE antibodies and mast cells . Arch Ophthalmol 1981;99:1831-1840.Article
18.
Mackenzie CD:  Eosinophil leukocytes in filarial infections . Trans R Soc Trop Med Hyg 1980;74( (suppl) ):51-58.Article
19.
Rodger FC:  Pathogenesis and pathology of ocular onchocerciasis . Am J Ophthalmol 1960; 49:560-594.
20.
Anderson J, Font RL:  Ocular onchocerciasis , in Binford CH, Connor DH (eds): Pathology of Tropical and Extraordinary Diseases . Washington, DC, Armed Forces Institute of Pathology, 1976, pp 373-377.
21.
Paul EV, Zimmerman LE:  Some observations on the ocular pathology of onchocerciasis . Hum Pathol 1970;1:581-594.Article
22.
Garner A:  Pathology of ocular onchocerciasis: Human and experimental . Trans R Soc Trop Med Hyg 1976;70:374-377.Article
23.
Thylefors B, Tønjum AM:  Visual field defects in onchocerciasis . Br J Ophthalmol 1978; 62:462-467.Article
24.
Jones BR, Anderson J, Fuglsang H:  Effects of various concentrations of diethylcarbamazine citrate applied as eye drops in ocular onchocerciasis, and the possibilities of improved therapy from continuous non-pulsed delivery . Br J Ophthalmol 1978;62:428-439.Article
25.
Martinez-Palomo A:  Ultrastructural characterization of the cuticle of Onchocerca volvulus microfilariae . J Parasitol 1978;64:127-136.Article
26.
Gibson DW, Connor DH, Brown HL, et al:  Onchocercal dermatitis: Ultrastructural studies of microfilariae and host tissues, before and after treatment with diethylcarbamazine . Am J Trop Med Hyg 1976;25:74-87.
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