[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address Please contact the publisher to request reinstatement.
[Skip to Content Landing]
September 1984

Quantitative Adherence of Bacteria to Human Corneal Epithelial Cells

Author Affiliations

From the Department of Ophthalmology, University of Florida College of Medicine, Gainesville.

Arch Ophthalmol. 1984;102(9):1394-1395. doi:10.1001/archopht.1984.01040031136041

• An in vitro assay was used to quantitatively determine the ability of a variety of bacteria to adhere to human corneal epithelial cells. Staphylococcus aureus, Streptococcus pneumoniae, and Pseudomonas aeruginosa were found to adhere to corneal epithelium significantly better than other species that were tested. However, there was considerable variability in the adherence of individual strains of these bacterial species. The results of this study support the hypothesis that the frequency with which certain species of bacteria cause bacterial corneal ulceration may be related to the ability of those organisms to adhere to corneal epithelium. In addition, differences in pathogenicity between strains of a given species may be related to the variability of adherence within a species.

Jones DB:  Early diagnosis and therapy of bacterial corneal ulcers . Int Ophthalmol Clin 1973;13:1-29.Article
Liesegang TJ, Forster RK:  Spectrum of microbial keratitis in south Florida . Am J Ophthalmol 1980;90:38-47.
Jones DB:  Initial therapy of suspected microbial corneal ulcers: II. Specific antibiotic therapy based on corneal smears . Surv Ophthalmol 1979;24:97.Article
Ostler HB, Okumoto M, Wilkey C:  The changing pattern of the etiology of central bacterial corneal (hypopyon) ulcer . Trans Pac Coast Otoophthalmol Soc 1976;57:235-246.
Reed WP, Williams RC Jr:  Bacterial adherence: First step in pathogenesis of certain infections . J Chronic Dis 1978;31:67-72.Article
Ofek I, Beachey EH:  Bacterial adherence . Adv Intern Med 1980;25:503-532.
Ramphal R, McNeice MT, Polack FA:  Adherence of Ps aeruginosa to the injured cornea: A step in the pathogenesis of corneal infections . Ann Ophthalmol 1981;13:421-425.
Stern GA, Weitzenkorn D, Valenti J:  Adherence of Pseudomonas aeruginosa to the mouse cornea: Epithelial v stromal adherence . Arch Ophthalmol 1982;100:1956-1958.Article
Hyndiuk RA:  Experimental Pseudomonas keratitis . Trans Am Ophthalmol Soc 1981;79:541-624.
Daniel WW: Biostatistics: A Foundation for Analysis in the Health Sciences . New York, John Wiley & Sons Inc, 1978, p 220.
Ellen RP, Gibbons RJ:  Parameters affecting the adherence and tissue tropisms of Streptococcus pyogenes . Infect Immun 1974;9:85-91.
Johnson AP, Wood BM, Johnson MK:  Adherence of S aureus to rabbit corneal epithelial cells . Invest Ophthalmol Vis Sci 1983;24( (suppl) ):195.