[Skip to Content]
[Skip to Content Landing]
Article
February 1985

An Antimelanoma Monoclonal Antibody and the Histopathology of Uveal Melanomas

Author Affiliations

From the Departments of Pathology (Dr Folberg and Ms Arbizo) and Research (Dr Donoso), Wills Eye Hospital and Thomas Jefferson University; the Department of Pathology and Laboratory Medicine, University of Pennsylvania (Drs Atkinson and Ernst); and the Wistar Institute of Anatomy and Biology (Drs Atkinson and Herlyn), Philadelphia. Dr Folberg is now with the University of Iowa Hospitals and Clinics, Iowa City.

Arch Ophthalmol. 1985;103(2):275-279. doi:10.1001/archopht.1985.01050020127035
Abstract

• Monoclonal antibody ME491 identifies a cutaneous melanoma-associated antigen in formaldehyde-fixed, paraffinembedded tissues. This antibody was applied to tissue sections from 79 cases of formaldehyde-fixed, paraffin-embedded uveal melanomas. Sixty-nine (87.3%) of the 79 cases showed staining by monoclonal antibody ME491, thus demonstrating an antigen shared by cutaneous and uveal melanomas. No relationship between the staining pattern and the patient outcome was detected. Twelve (85.7%) of the 14 cases in which balloon cells were present stained with the antibody. The antibody stained the long posterior ciliary nerve in 12 (38.7%) of 31 cases in which the nerve was present in tissue sections. The antibody cross reacted with the retinal pigment epithelium (16.5% of cases) and a variety of normal nonocular tissues.

References
1.
Neville AM, Foster CS, Moshakis V, et al:  Monoclonal antibodies and human tumor pathology . Hum Pathol 1982;13:1067-1081.Article
2.
Carrell S, Accolla RS, Carmagnoler AL, et al:  Common human melanoma-associated antigens detected by monoclonal antibodies . Cancer Res 1980;40:2523-2528.
3.
Dippold WG, Lloyd KO, Li LT, et al:  Cell surface antigens of human malignant melanoma: Definition of six antigenic systems with mouse monoclonal antibodies . Proc Natl Acad Sci USA 1980;77:6114-6118.Article
4.
Herlyn M, Clark WH, Mastrangelo M, et al:  Specific immunoreactivity of hybridoma-secreted monoclonal antimelanoma antibodies to cultured cells and freshly derived human cells . Cancer Res 1980;40:3602-3609.
5.
Herlyn M, Steplewski Z, Herlyn D, et al:  Production and characterization of monoclonal antibodies against human malignant melanoma . Cancer Invest 1983;12:127-136.
6.
Garrigues HJ, Tilgin W, Helstrom I, et al:  Detection of a human melanoma-associated antigen p97 in histological sections of primary human melanoma . Int J Cancer 1982;28:511-515.Article
7.
Herlyn M, Steplewski Z, Atkinson BF, et al:  Comparative study of the binding characteristics of monoclonal anti-melanoma antibodies . Hybridoma 1982;1:403-411.Article
8.
Atkinson BF, Ernst CS, Ghrist BFD, et al:  Identification of melanoma associated antigens using fixed tissue screening of antibodies . Cancer Res 1984;44:2577-2581.
9.
Folberg R, Donoso LA, Herlyn M, et al:  Antigens in ocular and cutaneous melanoma . Am J Ophthalmol 1983;96:394-395.
10.
Hsu SM, Raine L:  Protein A, avidin and biotin in immunohistochemistry . J Histochem Cytochem 1981;29:1349-1353.Article
11.
Nakajima T, Watanabe S, Sato Y, et al:  Immunohistochemical demonstration of S-100 protein in malignant melanoma and its diagnostic application . Cancer 1982;50:912-918.Article
12.
Cochran AJ, Wen DR, Hershman HR, et al:  Detection of S-100 protein as an aid to the identification of melanocytic tumors . Int J Cancer 1982;30:295-297.Article
13.
Folberg R, Donoso LA:  Monoclonal antibodies and ocular melanoma: II. Application to pathology , abstracted. Invest Ophthalmol Vis Sci Suppl 1984;25:82.
14.
Jakobiec FA, Shields JA, Desjardins L, et al:  Balloon cell melanomas of the ciliary body . Arch Ophthalmol 1979;97:1687-1692.Article
15.
Dvorak-Theobald G:  Neurogenic origin of choroidal sarcoma . Arch Ophthalmol 1937;18:971-997.Article
16.
Fine BS, Yanoff M: Ocular Histology . Hagerstown, Md, Harper & Row Publishers Inc, 1979, p 68.
17.
Elner VM, Schaffner T, Taylor K, et al:  Immunophagocytic properties of retinal pigment epithelial cells . Science 1981;211:74-76.Article
18.
Luna LG: Manual of Histologic Staining of the Armed Forces Institute of Pathology . New York, McGraw-Hill Book Co, 1968, p 48.
19.
Sheehan DC, Hrapchak BB: Theory and Practice of Histotechnology . St Louis, CV Mosby Co, 1980, p 221.
20.
Hsu SM, Soban E:  Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry . J Histochem Cytochem 1982; 30:1079-1082.Article
21.
Donoso LA, Folberg R, Herlyn M, Atkinson B:  Monoclonal antibodies and ocular melanoma , abstracted. Hybridoma 1984;3:94.
22.
Epstein J, Albert D, Ruzzo M, et al:  A method for cultivation of human ocular melanoma cells , abstracted. Invest Ophthalmol Vis Sci Suppl 1984;25:84.
23.
Waldrep JC, Kaplan HJ, Jarrett WH, et al:  Establishment of a human choroidal melanoma cell line , abstracted. Invest Ophthalmol Vis Sci Suppl 1984;25:209.
×