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May 1985

Lipid Keratopathy in RabbitsAn Animal Model System

Author Affiliations

From the Departments of Ophthalmology (Drs Stock, Mendelsohn, O'Grady, and Lo) and Biochemistry (Dr Ghosh), Northwestern University Medical School, and the Department of Ophthalmology, Veterans Administration Lakeside Medical Center (Dr Stock), Chicago.

Arch Ophthalmol. 1985;103(5):726-730. doi:10.1001/archopht.1985.01050050118030

• Rabbits rendered hypercholesterolemic on a high cholesterol diet while subjected to corneal suture placement to induce neovascularization made useful models for qualitatively and quantitatively studying lipid keratopathy. Forty rabbit eyes were subjected to placement of four sutures located between 1 and 4 mm from the limbus. The neovasculature grew at a constant rate of 0.24 mm/day, unaffected by serum cholesterol levels. Rabbits fed cholesterol prior to surgery were the earliest to display lipid keratopathy, followed by those who began cholesterol feedings on the day of surgery, and last by rabbits who began receiving high cholesterol food seven days following suture placement. A description of the corneal neovascularization process and histopathological and biochemical analyses of the induced lipid keratopathy are presented.

Jack JL, Luse RA:  Lipid keratopathy: An electron microscopic study . Arch Ophthalmol 1970;83:678-691.Article
Smolin G, Thoft RA: The Cornea: Scientific Foundations and Clinical Practice . Boston, Little Brown & Co, 1983, p 333.
Cogan DG, Kuwabara T:  Ocular changes in experimental hypercholesterolemia . Arch Ophthalmol 1959;61:219-225.Article
Klintworth GK, Garner A: Pathobiology of Ocular Disease: A Dynamic Approach , New York, Marcel Dekker Inc, 1982, pp 1444-1450.
Francois J, Neetens A:  The ocular manifestations of experimental hypercholesteraemia in rabbits . Ophthalmologica 1964;148:57-79.Article
Cogan DG, Kuwabara T:  Lipid keratopathy and atheroma . Circulation 1958;18:519-525.Article
Thoft RA, Friend J, Murphy HS:  Ocular surface epithelium and vascularization in rabbits . Invest Ophthalmol Vis Sci 1979;18:85-92.
Klintworth GK:  The contribution of morphology to our understanding of the pathogenesis of experimentally produced corneal vascularization . Invest Ophthalmol Vis Sci 1977;16:281-285.
Klintworth GK, Burger PC:  Neovascularization of the cornea: Current concepts of its pathogenesis . Int Ophthalmol Clin 1983;23( (1) ):27-39.Article
Langham M:  Observations on the growth of blood vessels into the cornea: Application of a new experimental technique . Br J Ophthalmol 1953;37:210-222.Article
Ashton N, Cook C:  Mechanism of corneal vascularization . Br J Ophthalmol 1953;37:193-208.Article
Campbell FW, Michaelson IC:  Blood vessel formation in the cornea . Br J Ophthalmol 1949; 33:248-255.Article
Ruben M:  Corneal vascularization . Int Ophthalmol Clin 1981;21( (2) ):27-38.Article
Federman JL, Brown GC, Felberg NT, et al:  Experimental ocular angiogenesis . Am J Ophthalmol 1980;89:231-237.
McAuslan BR, Gole GA:  Cellular and molecular mechanisms in angiogenesis . Trans Ophthalmol Soc UK 1980;100:354-358.
Groden LR, Cassel GH, Laibson PR:  The effect of corneal trephination on neovascularization . Ophthalmic Surg 1983;14:954-956.
Cogan DG:  Corneal vascularization . Invest Ophthalmol 1962;1:253-261.
Schanzlin DJ, Cyr RJ, Friedlaender MH:  Histopathology of corneal neovascularization . Arch Ophthalmol 1983;101:472-474.Article
Ross AC, Zilversmit DB:  Chylomicron remnant cholesteryl esters as the major constituent of very low density lipoproteins in plasma of cholesterol-fed rabbits . J Lipid Res 1977;18:169-181.
Ausprunk DH, Falterman K, Folkman J:  The sequence of events in the regression of corneal capillaries . Lab Invest 1978;38:284-294.
Cherry PMH, Garner A:  Corneal neovascularization treated with argon laser . Br J Ophthalmol 1976;60:464-472.Article
Ben Ezra D:  Neovasculogenic ability of prostaglandins, growth factors, and synthetic chemoattractants . Am J Ophthalmol 1978;86:455-461.
Ben Ezra D:  Possible mediation of vasculogenesis by products of immune reaction , in Silverstein AM, O'Connor GR (eds): Immunopathology of the Eye . New York, Masson Publishing USA Inc, 1979, pp 315-318.
Conn H, Berman M, Kenyon K, et al:  Stromal vascularization prevents corneal ulceration . Invest Ophthalmol Vis Sci 1980;19:362-370.
Walton KW, Dunkerly DJ:  Studies on the pathogenesis of corneal arcus formation: II. Immunofluorescent studies on lipid deposition in the eye of lipid-fed rabbit . J Pathol 1974;114:217-229.Article
Terpstra AHM, Sanchez-Muniz FJ:  Time course of the development of hypercholesterolemia in rabbits fed simipurified diets containing casein or soybean protein . Artherosclerosis 1981;39:217-227.Article