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April 1986

A Medium-Term Corneal Preserving Medium (K-Sol)

Author Affiliations

From the Lions Eye Research Laboratories, LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans. Dr Yau is now with Mackay Memorial Hospital, Taipei, Taiwan.

Arch Ophthalmol. 1986;104(4):598-601. doi:10.1001/archopht.1986.01050160154032

• A new corneal preserving solution, K-Sol, was prepared by combining TC 199, 100 mg/L of gentamicin sulfate, 0.025M HEPES buffer to a pH of 7.4, and various concentrations (2.0%; 2.25%; 2.5%) of chondroitin sulfate to a final osmolality of 310 mOsm. Rabbit corneas stored at 4° C for 12 days in each solution, as well as fresh controls, were used for penetrating keratoplasty. Three weeks later, one graft in the 2.0% group was opacified; the rest were clear and showed minimal thickening. There were no significant differences between controls in terms of pachymetry and endothelial cell loss. This study supports the finding that K-Sol with 2.5% chondroitin sulfate is a simple, effective, mediumterm preserving solution for donor tissue for penetrating keratoplasty in rabbits.

McCarey BE, Kaufman HE:  Improved corneal storage . Invest Ophthalmol Vis Sci 1974; 13:165-173.
McCarey BE, Meyer RF, Kaufman HE:  Improved corneal storage for penetrating keratoplasties in humans . Ann Ophthalmol 1976;8:1488-1495.
Capella JA:  Techniques of corneal cryopreservation , in Capella JA, Edelhauser HF, Van Horn DL (eds): Corneal Preservation . Springfield, Ill, Charles C Thomas Publisher, 1973, pp 308-317.
Kaufman HE:  Corneal cryopreservation and its clinical application . Transplant Proc 1976;8( (suppl 1) ):149-152.
Doughman DJ, Harris JE, Mindrup E, et al:  Prolonged donor corneal preservation in organ culture: Long-term clinical evaluation . Cornea 1982;1:7-20.
Kaufman HE, Varnell ED, Kaufman S:  Chondroitin sulfate in a new corneal preservation medium . Am J Ophthalmol 1984;98:112-114.Article
Kaufman HE, Varnell ED, Kaufman S, et al:  K-Sol corneal preservation . Am J Ophthalmol 1985;100:299-304.
Blackwell WL, Gravenstein N, Kaufman HE:  Comparison of central corneal endothelial cell numbers with peripheral areas . Am J Ophthalmol 1977;84:473-476.
Van Horn DL, Sendele DD, Seideman S, et al:  Regenerative capacity of the corneal endothelium in rabbit and cat . Invest Ophthalmol Vis Sci 1977;16:597-613.
Condon PI, Fitzgerald G, Burke A, et al:  The physical effects of viscoelastic substances on human donor cornea . Trans Ophthalmol Soc UK 1983;103:265-267.
Harrison SE, Soll DB, Shayegan M, et al:  Chondroitin sulfate: A new and effective protective agent for intraocular lens insertion . Ophthalmology 1982;89:1254-1260.Article
MacRae SM, Edelhauser HF, Hyndiuk RA, et al:  The effects of sodium hyaluronate, chondroitin sulfate, and methylcellulose on the corneal endothelium and intraocular pressure . Am J Ophthalmol 1983;95:332-341.
Carty JB:  Chondroitin sulfate in anterior segment surgery . Trans Ophthalmol Soc UK 1983; 103:263-264.
Ng MC, Riley MV:  Relation of intracellular levels and redox state of glutathione to endothelial function in the rabbit cornea . Exp Eye Res 1980;30:511-517.Article
Edelhauser HF, Van Horn DL, Miller P, et al:  Effect of thiol-oxidation of glutathione with diamide on corneal endothelial function, junctional complexes, and microfilaments . J Cell Biol 1976;68:567-578.Article
Hull DS, Green K, Bowman K, et al:  Intracellular pH and glutathione levels in rabbit corneal endothelium following storage in moist chamber and MK medium . Invest Ophthalmol Vis Sci 1983;24:214-217.
Trawkin AG, Gundorowa RA, Bordjugowa GG, et al:  Erforschung des Zustandes der Zellmembran und der Mechanismen der Hornhautautolyse bei der Konservierung . Klin Monatsbl Augenheilkd 1976;169:500-504.
Hull DS, Strickland EC, Green K:  Photodynamically induced alteration of cornea endothelial cell function . Invest Ophthalmol Vis Sci 1979;18:1226-1231.
Edelhauser HF, Hanneken AM, Pederson HJ, et al:  Osmotic tolerance of rabbit and human corneal endothelium . Arch Ophthalmol 1981; 99:1281-1287.Article
Gonnering R, Edelhauser HF, Van Horn DL, et al:  The pH tolerance of rabbit and human corneal endothelium . Invest Ophthalmol Vis Sci 1979;18:373-390.