[Skip to Content]
[Skip to Content Landing]
November 1986

DNA Content Abnormalities and Prognosis in Uveal Melanoma

Author Affiliations

From the Department of Ophthalmology and the Francis I. Proctor Foundation, University of California at San Francisco. Dr Char is an Alcon Institute Research Professor of Ophthalmology.

Arch Ophthalmol. 1986;104(11):1626-1629. doi:10.1001/archopht.1986.01050230064033

• Recent advances in flow cytometry allow for study of DNA content in paraffin-embedded pathology material. Using refinements of published techniques, we retrospectively correlated tumor cell DNA content (ploidy) with histologic findings and clinical outcome in 79 patients with uveal melanoma. Patients were included using these selection criteria: (1) enucleation without adjunctive therapy performed at the University of California at San Francisco between 1956 and 1979, (2) tumor located in ciliary body or choroid, and (3) pathology material and complete follow-up data available. The DNA histograms were classifiable as diploid or hyperploid in 64 cases. The mean coefficient of variation for diploid histograms was 6.6%. Twenty-three patients (36%) had hyperploid tumors. Hyperploidy was correlated with worse outcome. The effect was most marked for DNA indexes of over 1.4. A Cox proportional hazards model showed that the effects of tumor diameter and cell type were insignificant in comparison with the effect of the DNA index.

Li CH, Yieh MS, McInnis BC, et al:  Application of the estimation of normal distributions from truncated samples to the analysis of flow microfluorometry histograms . Comput Biomed Res 1982;15:262-271.Article
Friedlander ML, Taylor IW, Russell P, et al:  Ploidy as a prognostic factor in ovarian cancer . Int J Gynecol Pathol 1983;2:55-63.Article
Baisch H, Otto U, Konig K, et al:  DNA content of human kidney carcinoma cells in relation to histological grading . Br J Cancer 1982;45:878-885.Article
Friedlander ML, Hedley DW, Taylor IW, et al:  Influence of cellular DNA content on survival in advanced ovarian carcinoma . Cancer Res 1984; 44:397-400.
Volm M, Mattern J, Sonka J, et al:  DNA distribution in non-small-cell lung carcinoma and its relationship to clinical behavior . Cytometry 1985;6:348-356.Article
Frankfurt OS, Chin JL, Englander LS, et al:  Relationship between DNA ploidy, glandular differentiation, and tumor spread in human prostate cancer . Cancer Res 1985;45:1418-1423.
Hedley DW, Rugg CA, Ng AB, et al:  Influence of cellular DNA content on disease-free survival of stage II breast cancer patients . Cancer Res 1984;44:5395-5398.
Sondergaard K, Larsen JK, Moller OU, et al:  DNA ploidy-characteristics of human malignant melanoma analyzed by flow cytometry and compared with histology and clinical course . Virchows Arch Cell Pathol 1983;42:43-52.
Hedley DW, Freidlander ML, Taylor IW, et al:  The method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry . J Histochem Cytochem 1985;31:1333-1335.Article
Jensen OA:  Malignant melanomas of the human uvea: Recent follow-up of cases in Denmark, 1943-1952 . Acta Ophthalmol 1970;48:1113-1128.Article
Gamel JW, McLean IW, Greenberg RA, et al:  Computerized histologic assessment of malignant potential: A method for determining the prognosis of uveal melanomas . Hum Pathol 1982; 13:893.Article
Barlogie B, Hittelman W, Spitzer G, et al:  Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma . Cancer Res 1977;37:4400-4407.
Barlogie B, Raber MN, Schumann J, et al:  Flow cytometry in clinical cancer research . Cancer Res 1983;43:3982-3997.
Buchner T, Hiddemann W, Wormann B, et al:  Differential pattern of DNA-aneuploidy in human malignancies . Pathol Res Pract 1985; 179:310-317.Article
Barlogie B, Drewinko B, Schumann J, et al:  Cellular DNA content as a marker of neoplasia in man . Am J Med 1980;69:195-203.Article
Vindelov LL, Christensen IJ, Nissen NI:  A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis . Cytometry 1983;3:323-327.Article
Schutte B, Reynders MM, Bosman FT, et al:  Flow cytometric determination of DNA ploidy in nuclei isolated from paraffin-embedded tissue . Cytometry 1985;6:26-30.Article
Ban Driel-Kulker AMJ, Mesker WE, Van Velzen I, et al:  Preparation of monolayer smears from paraffin-embedded tissue for image cytometry . Cytometry 1985;6:268-272.Article
Fraschini A, Pellicciari C, Biggiogera, M, et al:  The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches . Histochem J 1981;13:763-779.Article
Barlogie B, Gohde W, Johnston DA, et al:  Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytofluorometry . Cancer Res 1978;38:3333-3339.
Teodori L, Tirnindelli-Danesi D, Mauro F, et al:  Non-small-cell lung carcinoma: Tumor characterization on the basis of a flow cytometrically determined cellular heterogeneity . Cytometry 1983;4:174-183.Article
Char DH, Juster RP, Heilbron DC, et al:  Choroidal melanoma growth patterns . Br J Ophthalmol 1983;67:575-578.Article
Juster RP, Char DH:  Uveal melanoma: Doubling time and mitotic index . Arch Ophthalmol 1986;104:174.Article