[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.205.209.213. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
February 1988

Iontophoresis of Tobramycin for the Treatment of Experimental Pseudomonas Keratitis in the Rabbit

Author Affiliations

From the Lions Eye Research Laboratories, LSU Eye Center (Drs Rootman and Hill, Ms Jantzen, and Mr Gonzalez) and the Department of Microbiology (Dr O'Callaghan and Mr Hobden), Louisiana State University Medical Center School of Medicine, New Orleans.

Arch Ophthalmol. 1988;106(2):262-265. doi:10.1001/archopht.1988.01060130276043
Abstract

• Iontophoresis of tobramycin sulfate was employed to treat bacterial keratitis induced in rabbits by an intrastromal injection of Pseudomonas aeruginosa. Quantitation of bacterial killing was achieved by culturing corneal homogenates and counting viable bacteria per cornea after treatment. When the rabbits received two iontophoretic treatments, iontophoresis significantly reduced the number of bacterial colony-forming units, compared with the use of an eyecup without current or two subconjunctival injections of 20 mg of tobramycin sulfate, and with untreated controls. The results of iontophoresis were not significantly different from those involving treatment with topical fortified drops (40 mg/mL). A single iontophoresis treatment was compared with the use of an eyecup without current or less fortified topical drops (13.6 mg/mL), as well as with untreated controls. Iontophoresis was significantly better than either the eyecup or the topical fortified tobramycin drops in reducing viable bacteria in the corneas. Iontophoresis may be a useful adjunct in the treatment of severe corneal bacterial infections.

References
1.
Laibson PR:  Annual review, cornea and sclera . Arch Ophthalmol 1972;88:553-574.Article
2.
Krachmer JH, Purcell JJ:  Bacterial corneal ulcers in cosmetic soft contact lens wearers . Arch Ophthalmol 1978;96:57-61.Article
3.
Eichenbaum JW, Feldstein J, Podos SM:  Extended-wear aphakic soft contact lenses and corneal ulcers . Br J Ophthalmol 1982;92:384-390.
4.
Adams CP, Cohen EJ, Laibson PR, et al:  Corneal ulcers in patients with cosmetic extended-wear contact lenses . Am J Ophthalmol 1983;96:705-709.
5.
Baum JL, Jones DB:  Initial therapy of suspected microbial corneal ulcers . Surv Ophthalmol 1979;24:97-116.Article
6.
Leibowitz HM, Ryan WJ, Kupferman A:  Route of antibiotic administration in bacterial keratitis . Arch Ophthalmol 1981;99:1420-1423.Article
7.
Davis SD, Sarff LD, Hyndiuk RA:  Comparison of therapeutic routes in experimental Pseudomonas keratitis . Am J Ophthalmol 1979;87:710-716.
8.
Baum JL, Barza M:  Topical vs subconjunctival treatment of bacterial corneal ulcers . Ophthalmology 1983;90:162-168.Article
9.
Hyndiuk RA:  Experimental Pseudomonas keratitis . Trans Am Ophthalmol Soc 1981;79:541-624.
10.
Hyndiuk RA, Skorich DN, Davis SD:  Fortified antibiotic ointment in Pseudomonas keratitis . Invest Ophthalmol Vis Sci 1987;28( (suppl) ):175.
11.
Gibson LE, Cooke RE:  A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis . Pediatrics 1959;23:545-549.
12.
Gangarosa LP, Hill JM, Thompson BL, et al:  Iontophoresis of vidarabine monophosphate for herpes orolabialis . J Infect Dis 1986;154:930-934.Article
13.
Gangarosa LP, Park NH:  Practical consideration in iontophoresis of fluoride for desensitizing dentin . J Prosthet Dent 1978;19:173-178.Article
14.
Gangarosa LP:  How modern iontophoresis can improve your practice . Quintessence Int 1982;10:1027-1038.
15.
Bridges MQM, Keene M, Graham JM, et al:  A device for iontophoretic anesthesia of the tympanic membrane . J Med Eng Technol 1982;6:62-64.Article
16.
Von Sallmann L:  Sulfadiazine iontophoresis in pyocyaneus infection of rabbit cornea . Am J Ophthalmol 1942;25:1292-1300.
17.
Von Sallmann L:  Penicillin and sulfadiazine in the treatment of experimental intraocular infection with Staphylococcus aureus and Clostridium welchii . Arch Ophthalmol 1944;31:54-61.Article
18.
Von Sallmann L:  Controversial points in ocular penicillin therapy . Trans Am Ophthalmol Soc 1944;83:1-67.
19.
Barza MM, Peckman C, Baum JL:  Transscleral iontophoresis of gentamicin in monkeys . Invest Ophthalmol Vis Sci 1987;28:1033-1036.
20.
Barza M, Peckman C, Baum J:  Transscleral iontophoresis as an adjunctive treatment for experimental endophthalmitis . Arch Ophthalmol 1987;105:1418-1420.Article
21.
Fishman PH, Jay WM, Hill JM, et al:  Iontophoresis of gentamicin into aphakic rabbit eyes: Sustained vitreal level . Invest Ophthalmol Vis Sci 1984;25:343-345.
22.
Hill JM, Haruta Y, Rootman DS:  Adrenergically induced recurrent HSV-1 corneal epithelial lesions . Curr Eye Res 1987;6:1065-1071.Article
23.
Hill JM, Kwon BS, Shimomura Y, et al:  Herpes simplex virus recovery in neural tissues after ocular HSV shedding induced by epinephrine iontophoresis to the rabbit cornea . Invest Ophthalmol Vis Sci 1983;24:243-227.
24.
Hill JM, Shimomura Y, Kwon BS, et al:  Iontophoresis of epinephrine isomers to rabbit eyes induced HSV-1 ocular shedding . Invest Ophthalmol Vis Sci 1985;26:1299-1303.
25.
Caudill JW, Romanowski E, Araullo-Cruz T, et al:  Recovery of a latent HSV-1 thymidine kinase negative strain following iontophoresis and co-cultivation in the ocularly infected rabbit model . Curr Eye Res 1986;5:41-45.Article
26.
Reller LB, Schoenknecht FD, Kenny MA, et al:  Antibiotic susceptibility testing of Pseudomonas aeruginosa: Selection of a control strain and criteria for magnesium and calcium content in media . J Infect Dis 1974;130:454-463.Article
27.
SAS Institute Inc: SAS Users Guide: Statistics Version , ed 5. Cary, NC, SAS Institute Inc, 1985.
28.
Oakley DE, Weeks RD, Ellis PP:  Corneal distribution of subconjunctival antibiotics . Am J Ophthalmol 1976;81:307-312.
29.
Barza M, Kane A, Baum JL:  Regional differences in ocular concentration of gentamicin after subconjunctival and retrobulbar injection in the rabbit . Am J Ophthalmol 1977;83:407-413.
×