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October 1989

Transforming Growth Factor Beta Does Not Work Without Angiogenesis In Vivo-Reply

Author Affiliations

Miami, Fla
Baltimore, Md

Arch Ophthalmol. 1989;107(10):1421. doi:10.1001/archopht.1989.01070020494011

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In Reply.  —We appreciate the opportunity to respond to the comments of Dr Beranek concerning our study. While we acknowledge the possibility that neovascularization may be induced by transforming growth factor beta,1 we are unable to identify the presence of retinal neovascularization in any of the experimental retinal lesions. In addition, we performed special stains using immunoperoxidase techniques for factor VIII (ie, neovascular tissue), which were also negative. Clearly, as Dr Beranek points out, angiogenesis is often an inherent part of wound repair throughout the body in general, but is not a component of retinal wound healing.2The size of a solid black region on an autoradiograph is not limited by the dimension of the individual interstitial cells since it represents a photographic exposure of an emulsion overlying the tissue. Therefore, with particularly "hot" cells, the autoradiograph can demonstrate a black mark of aggregated grains that exceeds the

Roberts AB, Sporn MB, Assaan RK, Smith NS, Roache LM, Wakefield UI.  Transforming growth factor type-beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro . Proc Natl Acad Sci USA . 1986;83:4167-4171.Article
Miller B, Miller H, Patterson R, Ryan SJ.  Effect of the vitreous on retinal wound healing . Graefes Arch Clin Exp Ophthalmol . 1986;224:576-579.Article