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—We appreciate the opportunity to respond to the comments of Dr Beranek concerning our study. While we acknowledge the possibility that neovascularization may be induced by transforming growth factor beta,1 we are unable to identify the presence of retinal neovascularization in any of the experimental retinal lesions. In addition, we performed special stains using immunoperoxidase techniques for factor VIII (ie, neovascular tissue), which were also negative. Clearly, as Dr Beranek points out, angiogenesis is often an inherent part of wound repair throughout the body in general, but is not a component of retinal wound healing.2The size of a solid black region on an autoradiograph is not limited by the dimension of the individual interstitial cells since it represents a photographic exposure of an emulsion overlying the tissue. Therefore, with particularly "hot" cells, the autoradiograph can demonstrate a black mark of aggregated grains that exceeds the
Smiddy WE, Glaser B, Green WR. Transforming Growth Factor Beta Does Not Work Without Angiogenesis In Vivo-Reply. Arch Ophthalmol. 1989;107(10):1421. doi:10.1001/archopht.1989.01070020494011