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April 1991

Synthesis of Extracellular Matrix by Macrophage-Modulated Retinal Pigment Epithelium

Author Affiliations

From the Department of Ophthalmology, University of Southern California School of Medicine, and the Doheny Eye Institute, Los Angeles, Calif. Dr Martini is recipient of a foreign fellowship grant from Research to Prevent Blindness Inc, New York, NY. Dr Martini is now with the Department of Ophthalmology, Karolinska Hospital and Institute, Stockholm, Sweden.

Arch Ophthalmol. 1991;109(4):576-580. doi:10.1001/archopht.1991.01080040144046

• In proliferative vitreoretinopathy, macrophages and retinal pigment epithelial cells are associated with microfibrillar matrix proteins in the vitreous cavity, but the contribution of this extracellular matrix to the pathophysiology is not known. We used radiolabeling techniques on cultured human retinal pigment epithelial cells to correlate the secretion of extracellular matrix proteins with macrophage-induced modulation of cell proliferation and morphologic features. Retinal pigment epithelial cells incubated in a macrophage-conditioned medium assumed fibrocytelike morphologic characteristics, grew faster, and exhibited a decreased cellular release of fibrillar and nonfibrillar matrix components. However, due to a simultaneous greater increase in cell numbers in these modulated cultures, the total production of fibrillar and nonfibrillar maxtrix components by the culture population was increased.

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