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Article
April 1996

Familial Uveal Melanoma: Absence of Constitutional Cytogenetic Abnormalities in 14 Cases

Author Affiliations

Philadelphia, Pa

Arch Ophthalmol. 1996;114(4):502-503. doi:10.1001/archopht.1996.01100130498042

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Abstract

The study of inherited cancer syndromes offers an opportunity to identify the genetic mechanisms of carcinogenesis. In familial cancers, detection of a constitutional cytogenetic abnormality such as deletion, inversion, or unbalanced translocation has provided clues to the location of the retinoblastoma predisposition gene, RB1, the neurofibromatosis type I gene, hereditary cutaneous melanoma gene, and others. The genetic basis of inheritance of uveal melanoma is not known, therefore, rare cases of familial uveal melanoma (FUM) offer a unique opportunity for investigation. As a first step for initiating molecular genetic studies, we performed karyotype of constitutional cells (peripheral blood lymphocytes) in 14 probands, derived from the 56 patients with FUM described in this issue of the Archives.1

We karyotyped constitutional cells by initiating cultures of peripheral blood lymphocytes in enriched RPMI-16 medium containing 10% fetal bovine serum, glutamine, gentamycin, and phytohemagglutinin. After 72 hours the cells were harvested with colcemid and

References
1.
Singh AD, Shields CL, De Potter P, et al.  Familial uveal melanoma: clinical observations on 56 patients . Arch Ophthalmol . 1996;114:392-399.Article
2.
Cannon-Albright LA, Goldgar DE, Meyer LJ, et al.  Assignment of a locus for familial melanoma, MLM, to chromosome 9p13-p22 . Science . 1992;258:1148-1151.Article
3.
Singh AD, Boghosian-Sell L, Wary KK, et al.  Cytogenetic findings in primary uveal melanoma . Cancer Genet Cytogenet . 1994;72:109-115.Article
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