Objective: CD4+CD25 high T cells containing natural regulatory T cells (nTreg) are present in tumors and blood of patients with head and neck squamous cell carcinoma (HNSCC) and suppress immune responses. Pure populations of nTreg are necessary to define their phenotypic and functional profile in patients with cancer.
Methods: CD4+CD25+ peripheral blood mononuclear cells (PBMC) of 12 patients with HNSCC were separated from CD4+CD25− cells using fluorescence-activated cell sorting. Flow cytometry was used to determine phenotypes. Responder CD4+CD25− cells stimulated with OKT3 were coincubated with autologous Treg in CFSE-based assays. CD4+CD25+ and CD4+CD25− T-cell subsets were expanded with or without rapamycin (1nM) with CD3/CD28-coated beads and IL-2. Annexin V binding was used to measure apoptosis of T cells.
Strauss L, Johnson JT, Whiteside TL. P094 Phenotypic and Functional Profiles of Natural Regulatory T Cells in the Circulation of Patients With Head and Neck Squamous Cell Carcinoma. Arch Otolaryngol Head Neck Surg. 2006;132(8):883-884. doi:10.1001/archotol.132.8.883-c