Objective: To understand the role of p53 in head and neck squamous cell carcinoma (HNSCC) cells in response to cisplatin treatment.
Design: The HNSCC cells that contain mutant (nonfunctional) p53 and high levels of the prosurvival protein Bcl-xL were transduced with wild-type (WT) p53, and HNSCC cells containing WT p53, also overexpressing Bcl-xL, were transduced with short interfering RNA p53 constructs. These transduced cell lines were compared with parental cell lines for sensitivity to cisplatin treatment.
Bauer JA, Brenner J, Sikora MJ, Bradford CR, Carey TE. P100 Genetic Alterations in p53 in Head and Neck Cancer Cell Lines Change Cisplatin Sensitivity. Arch Otolaryngol Head Neck Surg. 2006;132(8):885. doi:10.1001/archotol.132.8.885-b