Background: Saliva is a readily accessible secretion that may be used in the diagnosis and screening of patients with head and neck squamous cell carcinoma (HNSCC).
Methods: Saliva from 5 healthy volunteers and 3 patients with oral squamous cell carcinomas was used for proteomic analysis; we first depleted major proteins by affinity column. Depleted saliva was then divided and analyzed by the following methods: (1) liquid chromatography-tamden mass spectrometric (LC-MS/MS) after in-solution digestion and peptide anion exchange chromatography, (2) peptide separation by chromatography, and (3) sodium dodecyl sulfate–polyacrylamide gel electrophoresis and in-gel digestion and liquid chromatography/tandem mass spectrometry.
Ohshiro K, Kobayashi R, Clayman GL, Rosenthal DI, Weber RS, El-Naggar AK. P123 Proteomic Screening of Saliva in Head and Neck Squamous Carcinoma: A Potential Diagnostic and Screening Application. Arch Otolaryngol Head Neck Surg. 2006;132(8):889. doi:10.1001/archotol.132.8.889-a