Objective: To develop techniques for isolation, short-term culture, and in vivo modeling of epithelial and stromal cells from head and neck squamous cell carcinoma (HNSCC).
Design: Xenograft model trial.
Patients: Specimens of HNSCC were obtained from 34 patients (20 incident, 14 persistent or recurrent).
Interventions: Primary HNSCC tissue was disaggregated with trypsin and collagenase 1A. Cells were seeded onto collagen IV–coated tissue culture plates in keratinocyte growth media with fetal bovine serum, additives, and antibiotics. Serum was removed on day 3, and later media were switched to MEM/RPMI with fetal bovine serum. After short-term growth in culture, cells were transferred to denuded rat tracheas and implanted subcutaneously in nude mice.
Whigham AS, Netterville JL, Burkey BB, Sinard RJ, Chung CH, Slebos RJC, Yarbrough WG. P193 Short-term Culture and In Vivo Modeling of Primary Head and Neck Squamous Cell Carcinoma. Arch Otolaryngol Head Neck Surg. 2006;132(8):901-902. doi:10.1001/archotol.132.8.901-c