Objective: To assess the growth inhibitory activity of various intraoperative irrigation fluids on free squamous cell cancer (SCC) cells in an animal wound model simulating head and neck cancer surgery.
Design: Animal study with a cancer cell–contaminated wound model and in vitro tumor growth assay.
Subjects: C3H/HeJ mice and syngeneic SCC VII cells.
Interventions: After seeding 1 million SCC VII cells at each wound, we incubated the cells in situ for 30 minutes. The wounds were irrigated for 5 minutes with 5 different fluids: distilled water, 5% Betadine Solution (povidone-iodine), 3.2% hydrogen peroxide, isotonic sodium chloride (normal saline), and cisplatin. Tumor growth and wound healing were observed. Assays using 3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazoliumbromide (MTT) were performed to examine tumor growth inhibition in vitro with different concentrations and exposure times of Betadine Solution and distilled water.
Hah J, Jung Y, Kwon S, Kim K, Sung M. P206 Selection of Irrigation Fluid to Eradicate Free Cancer Cells During Head and Neck Cancer Surgery. Arch Otolaryngol Head Neck Surg. 2006;132(8):904-905. doi:10.1001/archotol.132.8.904-c