Objective: Malignant transformation of recurrent respiratory papillomatosis (RRP) is a very rare event. The disease in these patients shows a spectrum of morphologically discrete areas from papilloma to carcinoma. The aim of this study was to assess alterations in expression of human papillomavirus (HPV) types and molecular genetic markers in each distinct focus of papilloma and carcinoma.
Design: Morphologically distinct areas in pathology specimens were diagnosed as papilloma, dysplasia, and carcinoma. Using the laser capture microdissection technique, we separately obtained lesions within each specimen, and these were amplified by polymerase chain reaction to check for the presence of HPV. A DNA chip was used to determine the type of HPV in each area. Immunohistochemical analysis for p53, Ki-67, and pRb was performed.
Jeong W, Hah J, Jung Y, Jeon Y, Song Y, Kim K, Sung M. P208 A Molecular Study of Recurrent Respiratory Papillomatosis Undergoing Malignant Transformation. Arch Otolaryngol Head Neck Surg. 2006;132(8):905. doi:10.1001/archotol.132.8.905-b