Objective: To investigate the therapeutic efficacy of dendritic cell–tumor fusion hybrids against a murine squamous cell carcinoma (SCC)
Design: Squamous cell carcinoma VII is a poorly immunogenic murine SCC in C3H (H-2K) mice. Subdermal tumors were established by inoculation in the midabdomen of mice. Tumor diameter were measured with a Veneer caliper and used as an indication of treatment efficacy. Dendritic cells were generated from bone marrow cells, cultured with cytokines for 8 days, harvested, and mixed with cultured tumor cells in a 1:1 ratio. Cell fusion was achieved by exposing the cell mixture to an alternate electrical current to bring cells into alignment and close together, followed by a short direct electrical current pulse.
Lee WT, Tamai H, Cohen P, Shu S. P239 Immunotherapy of Established Murine Squamous Cell Carcinoma Using Dendritic Cell–Tumor Fusion Hybrids. Arch Otolaryngol Head Neck Surg. 2006;132(8):912. doi:10.1001/archotol.132.8.912-b