University of Pittsburgh Cancer Institute, Pittsburgh, Pa;
Altor BioScience Corporation, Miramar, Fla
Objective: We have shown that the human papillomavirus (HPV) E6 oncoprotein in HPV-16+ head and neck squamous cell carcinoma (HNSCC) cells induces proteasomal degradation of p53, which leads to greater CD8+ T-cell lysis mediated by enhanced expression of HLA-A2-p53(264-272) complexes on the tumor cell surface. By using a staining reagent capable of recognizing these complexes, we will be able to quantify and compare expression of HLA-A2p53(264-272) complexes in HPV-infected HNSCC cells and what the effect of p53 destabilization has on this expression.
Kim H, Zhu X, Lopez-Albaitero A, Sirianni N, Wong HC, Ferris RL. S004 HPV-Infected Cancers Process and Present HLA-A2–p53(264-272) Peptide Complexes: Implications for p53-Based Immunotherapy. Arch Otolaryngol Head Neck Surg. 2006;132(8):839. doi:10.1001/archotol.132.8.839-a