Fred Hutchinson Cancer Research Center, Seattle, Wash;
University of Washington, Seattle
Objective: We sought to determine if promoter methylation in tumor suppressor and cell adhesion genes can be correlated with tumor stage, nodal metastasis status, or human papillomavirus (HPV) infection of oral squamous cell carcinoma (OSCC).
Design: We tested the methylation status of 27 tumor suppressor and cell adhesion genes, including ADAM23, APC, ATM, CDH13, CDKN2A, CHFR, DAPK1, IGSF4, MLH1, PKP1, RARB, RASSF1, and TP73, and an additional gene, MAL, using the SALSA methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) tumor suppressor kit or methylation-specific polymerase chain reaction (MS-PCR) on bisulphite-treated DNA. χ2 Tests were used to determine statistical significance.
Houck J, Lohavanichbutr P, Yueh B, Mendez E, Futran N, Upton M, Schwartz SM, Chen C. S078 Promotor Methylation Status of Tumor Suppressor and Cell Adhesion Genes in Oral Squamous Cell Carcinomas. Arch Otolaryngol Head Neck Surg. 2006;132(8):856-857. doi:10.1001/archotol.132.8.856-c