Objective: Promoter methylation–mediated silencing is a hallmark of many established tumor suppressor genes. This study uses a novel, multigene approach to examine epigenetic events of aberrant promoter methylation as diagnostic markers in head and neck squamous cell carcinoma (HNSCC).
Design: DNA was interrogated for alterations in aberrant methylation status using the novel multigene (22 genes) methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay.
Sawhney R, Chen K, Khan M, Benninger MS, Worsham MJ. S079 Methylation of Multiple Genes as Diagnostic and Therapeutic Markers in Primary HNSCC. Arch Otolaryngol Head Neck Surg. 2006;132(8):857. doi:10.1001/archotol.132.8.857-a