Objective: To determine the contribution of reactive oxygen species (ROS) in (−)-gossypol-induced cell death in head and neck squamous cell carcinoma (HNSCC) cell lines.
Design: The HNSCC cell lines were treated with (−)-gossypol, and generation of ROS was measured by fluorescence microscopy and flow cytometry. Cells were treated with (−)-gossypol and/or antioxidant, or with (−)-gossypol that was pretreated with hydrogen peroxide. Following treatment, generation of ROS was measured, and cell survival was assayed.
Sikora MJ, Bauer JA, Brenner JC, Carey TE, Bradford CR. S110 (−)-Gossypol, a BH3 Mimetic and Inducer of Apoptosis in Head and Neck Cancer Cells, Is Oxidized and Deactivated by ROS. Arch Otolaryngol Head Neck Surg. 2006;132(8):863. doi:10.1001/archotol.132.8.863-b