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Article
June 1931

OTOSCLEROSISSTAINING AND CHEMICAL REACTIONS

Arch Otolaryngol. 1931;13(6):824-828. doi:10.1001/archotol.1931.04230040040003
Abstract

The peculiar staining reactions of the foci in otosclerosis have long attracted attention. Various ideas have been expressed to explain the striking bluish-red appearance such as occurs with the hematoxylin-eosin stain. A number of investigators have attributed the bluish reaction to the presence of calcium. It has been supposed that the focus has so great a content of calcium that even after decalcification some of it would still be present and would show on staining with hematoxylin. The blue staining has also been thought to be due to the peculiar chemical characteristics of the ground or cement substance in the foci. Just what the chemistry of this is does not appear to be known. Another view is that the increased supply of blood and lymph to the focus produces the blue stain. It is also believed that the pink-staining tissue usually found just underneath the osteoblasts that line the enlarged

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